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Products:Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs
Overview
Product name
Anti-VAMP1 antibody
See all VAMP1 products (8) ...
Description
Rabbit polyclonal to VAMP1
Tested applications
IHC-FoFr, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Cow, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat VAMP1.
(Peptide available as ab41454.)
Positive control
WB: This antibody gave a positive signal in the following tissue lysates: Mouse Brain, Mouse Spinal Cord, Rat Brain and Rat Spinal Cord. IHC: rat brain
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
- 1 Image
WB
Application notes
IHC-FoFr: 1/3000.
WB: Use at a concentration of 1 mg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Tissue specificity
Nervous system, skeletal muscle and adipose tissue.
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localization
Mitochondrion outer membrane; Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome and Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome.
Target information above from: UniProt accessionP23763
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686H12131 antibody
- SYB 1 antibody
- SYB1 antibody
see all
Database links
- Entrez Gene: 513621 Cow
- Entrez Gene: 6843 Human
- Entrez Gene: 22317 Mouse
- Entrez Gene: 25624 Rat
- Omim: 185880 Human
- SwissProt: Q0V7N0 Cow
- SwissProt: P23763 Human
- SwissProt: Q62442 Mouse
see all
Anti-VAMP1 antibody images:
Western blot - VAMP1 antibody (ab41324)
All lanes : Anti-VAMP1 antibody (ab41324) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate - normal tissue
Lane 3 : Spinal Cord (Mouse) Tissue Lysate
Lane 4 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (PFA perfusion fixed frozen sections) - VAMP1 antibody (ab41324)
Immunohistochemistical detection of VAMP1 with ab41324 on rat brain PFA perfusion-fixed frozen sections. Primary antibody ab41324 diluted at 1/3000 and incubated for 18 hours at 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 (1/1000)Immunostaining obtained in the rat hippocampus using this antibody diluted 1:1000. Sections come from a perfused-fixed animal. The immunostaining was performed using the `free floating` technique, using direct fluorescence. The antibody stains synapses in many brain areas. The picture shows the staining taken at low power magnification, showing the staining in the hippocampus (top) and medium thalamus (bottom of the picture).
Sophie Pezet, CNRS, Paris, France
References for Anti-VAMP1 antibody (ab41324)
ab41324 has not yet been referenced specifically in any publications.
Publishing research using ab41324? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"