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Western blot - VAMP1 antibody (ab41324)
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Immunohistochemistry (PFA perfusion fixed frozen sections) - VAMP1 antibody (ab41324)
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Product Name
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VAMP1 antibody
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See all VAMP1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to VAMP1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat VAMP1. (Peptide available as ab41454.)
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Reacts with
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Ms, Rat
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Predicted to react
(species key)
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Hu
(Due to sequence homology)
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Tested applications
(see key)
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IHC-FoFr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-FoFr: 1/3000.
WB: Use at a concentration of 1 mg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: This antibody gave a positive signal in the following tissue lysates:
Mouse Brain, Mouse Spinal Cord, Rat Brain and Rat Spinal Cord.
IHC: rat brain
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Cellular localization
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Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein
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Research areas
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Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs
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Relevance
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The Vesicle Associated Membrane Proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered. It has been shown that the extreme carboxy-terminus of VAMP1 is involved in subcellular vesicle targeting.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.400 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VAMP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VAMP1 antibody images, references, products related to ab41324 and other tools.
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VAMP1 antibody images:
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 Western blot - VAMP1 antibody (ab41324)
All lanes : VAMP1 antibody (ab41324) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate - normal tissue
Lane 3 : Spinal Cord (Mouse) Tissue Lysate
Lane 4 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (PFA perfusion fixed frozen sections) - VAMP1 antibody (ab41324)
Immunohistochemistical detection of VAMP1 with ab41324 on rat brain PFA perfusion-fixed frozen sections. Primary antibody ab41324 diluted at 1/3000 and incubated for 18 hours at 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 (1/1000)Immunostaining obtained in the rat hippocampus using this antibody diluted 1:1000. Sections come from a perfused-fixed animal. The immunostaining was performed using the `free floating` technique, using direct fluorescence. The antibody stains synapses in many brain areas. The picture shows the staining taken at low power magnification, showing the staining in the hippocampus (top) and medium thalamus (bottom of the picture). Sophie Pezet, CNRS, Paris, France
See Abreview
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Search PubMed (MEDLINE) for references to VAMP1
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VAMP1 antibody - more information
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Customer reviews (feedback) regarding VAMP1 antibody |
Customer FAQs regarding VAMP1 antibody |
Protocols for VAMP1 antibody |
Price and availability of products related to VAMP1 antibody |
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VAMP1 antibody - related products:
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VAMP1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about VAMP1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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