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Products:Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
Overview
Product name
Anti-VAMP2 antibody
See all VAMP2 products (10) ...
Description
Rabbit polyclonal to VAMP2
Tested applications
WB, IP, IHC-Fr, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Synthetic peptide: MSATAATVPPAAPAGEGG-C, corresponding to amino acids 1-18 of Rat VAMP2.
MSATAATVPP AAPAGEGG-C
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IP: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
ICC/IF: 1/1000
Target
Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Tissue specificity
Nervous system and skeletal muscle.
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
Target information above from: UniProt accessionP63027
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
see all
Database links
- Entrez Gene: 22318 Mouse
- Entrez Gene: 24803 Rat
- SwissProt: P63044 Mouse
- SwissProt: P63045 Rat
- Unigene: 28643 Mouse
- Unigene: 12939 Rat
Anti-VAMP2 antibody images:
Western blot - VAMP2 antibody (ab3347)
Predicted band size : 19 kDa
Western blot of VAMP-2 on rat brain extract using ab3347.
Immunocytochemistry/ Immunofluorescence - VAMP2 antibody (ab3347)
VAMP2 antibody (ab3347) used in Immunohistochemistry (rat frozen sections). Sections were fixed with paraformaldehyde. Primary Antibody ab3347 used at 1/1000 incubated for 18 hours at 20°C in PBS-T 0.3%. Secondary Antibody used anti-IgG Rabbit Conjugation Alexa Fluor® 488 Dilution 1/1000.
Karine Thibault, Laboratoire de Neurobiologie, Paris
References for Anti-VAMP2 antibody (ab3347)
This product has been referenced in:
- Han WQet al. SNARE-mediated rapid lysosome fusion in membrane raft clustering and dysfunction of bovine coronary arterial endothelium. Am J Physiol Heart Circ Physiol 301:H2028-37 (2011).Read more (PubMed: 21926345) »
- Zhu LQet al. GSK-3beta inhibits presynaptic vesicle exocytosis by phosphorylating P/Q-type calcium channel and interrupting SNARE complex formation. J Neurosci 30:3624-33 (2010). WB, IP; Rat.Read more (PubMed: 20219996) »
See all 7 publications for this product
Publishing research using ab3347? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"