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Product name
Anti-VAMP2 antibody
See all VAMP2 products (9) ...
Description
Rabbit polyclonal to VAMP2
Specificity
This antibody appears to be specific for VAMP2.
Tested applications
ICC/IF, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide from part of human, rat, mouse, xenopus (and many other species) VAMP2, conjugated to an immunogenic carrier protein
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
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Immunocytochemistry/ Immunofluorescence - VAMP2 antibody (ab68832)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab68832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
WB: Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 13 kDa.This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
IHC-P: Use a concentration of 1 - 10 µg/ml.
IHC-Fr: Use a concentration of 1 - 10 µg/ml.
Target
Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Tissue specificity
Nervous system and skeletal muscle.
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
Target information above from: UniProt accessionP63027
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
see all
Database links
- Entrez Gene: 6844 Human
- Entrez Gene: 22318 Mouse
- Entrez Gene: 24803 Rat
- Omim: 185881 Human
- SwissProt: P63027 Human
- SwissProt: P63044 Mouse
- SwissProt: P63045 Rat
- Unigene: 25348 Human
see all
Anti-VAMP2 antibody images:
Immunocytochemistry/ Immunofluorescence - VAMP2 antibody (ab68832)
ICC/IF image of ab68832 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68832, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-VAMP2 antibody (ab68832)
ab68832 has not yet been referenced specifically in any publications.
Publishing research using ab68832? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"