VAMP2 antibody (ab70222)

Product Name 

VAMP2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to VAMP2

Immunogen 

Synthetic peptide (Human, Rat, Mouse, Xenopus and other species) conjugated to an immunogenic carrier protein

Reacts with 
(species key)

Hu, Ms, Rat, Xl

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/2000.
IHC-P: 1/300 - 1/3000. Antigen retrieval is recommended.
IHC-Fr: 1/300 - 1/3000.
WB: 1/300 - 1/3000. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

1 day old mouse cortical cell culture
Rat brain lysate
Rat brain cortex

Cellular localization 

Cytoplasmic vesicle; secretory vesicle; synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs

Relevance 

VAMP2 (Synaptobrevin 2) is a small integral membrane protein found specifically in synaptic vesicles in neurons. Synaptobrevins/VAMPs, syntaxins, and the 25 kD synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane.
VAMP2 is one of a family of integral membrane proteins of 11-13 kDa with the N terminal region exposed to the cytoplasm and a C terminal transmembrane domain. Two isoforms identified in the mammalian CNS Synaptobrevin 1/VAMP 1 and Synaptobrevin 2/VAMP 2 differ in their distribution within different brain regions. Synaptobrevin/VAMP is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin 2/VAMP 2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells, the third isoform, Cellubrevin/VAMP 3, is present where it is localized to an endosomal membrane pool. Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C terminal portion of the molecule.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole serum

Form 

Lyophilised
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.

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