Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
Overview
Product name
Anti-VAMP2 antibody
See all VAMP2 products (10) ...
Description
Rabbit polyclonal to VAMP2
Tested applications
IHC-P, IHC-Fr, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Xenopus laevis
Immunogen
Synthetic peptide (Human, Rat, Mouse, Xenopus and other species) conjugated to an immunogenic carrier protein
Positive control
1 day old mouse cortical cell culture Rat brain lysate Rat brain cortex
Properties
Form
Lyophilised:
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Concentration
Concentration information loading...
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/2000.
IHC-P: 1/300 - 1/3000. Antigen retrieval is recommended.
IHC-Fr: 1/300 - 1/3000.
WB: 1/300 - 1/3000. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Tissue specificity
Nervous system and skeletal muscle.
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
Target information above from: UniProt accessionP63027
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
see all
Database links
- Entrez Gene: 6844 Human
- Entrez Gene: 22318 Mouse
- Entrez Gene: 24803 Rat
- Entrez Gene: 394287 Xenopus laevis
- Omim: 185881 Human
- SwissProt: P63027 Human
- SwissProt: P63044 Mouse
- SwissProt: P63045 Rat
see all
Anti-VAMP2 antibody images:
Immunocytochemistry/ Immunofluorescence - VAMP2 antibody (ab70222)
Immunofluorescence analysis of 1 day old mouse cortical cell culture using ab70222 at 1/2000 dilution.
Western blot - VAMP2 antibody (ab70222)
All lanes : Anti-VAMP2 antibody (ab70222) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat brain lysate with immunizing peptide
Predicted band size : 13 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Frozen sections) - VAMP2 antibody (ab70222)
Immunohistochemistry of rat brain cortex using ab70222 at 1/1000 dilution.
References for Anti-VAMP2 antibody (ab70222)
This product has been referenced in:
- Huang Get al. a3(V) Collagen is critical for glucose homeostasis in mice due to effects in pancreatic islets and peripheral tissues. J Clin Invest : (2011). WB; Mouse.Read more (PubMed: 21293061) »
- Liew CWet al. The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells. J Clin Invest 120:2876-88 (2010). WB; Human.Read more (PubMed: 20592469) »
See all 2 publications for this product
Publishing research using ab70222? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"