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Product name
Anti-VAMP2 antibody
See all VAMP2 products (9) ...
Description
Rabbit polyclonal to VAMP2
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat VAMP2.
(Peptide available as ab916 13.)
Positive control
This antibody gave a positive signal in the following rat tissue lysates: Brain; Spinal cord; Cerebellum; Hippocampus; Forebrain; Cortex as well as PC12 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 12 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Tissue specificity
Nervous system and skeletal muscle.
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
Target information above from: UniProt accessionP63027
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
see all
Database links
Anti-VAMP2 antibody images:
Western blot - VAMP2 antibody (ab82507)
All lanes : Anti-VAMP2 antibody (ab82507) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Spinal Cord (Rat) Tissue Lysate
Lane 3 : Cerebellum (Rat) Tissue Lysate
Lane 4 : Forebrain (Rat) Tissue Lysate
Lane 5 : Hippocampus (Rat) Tissue Lysate
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 7 : Cortex (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 12 kDa
Observed band size : 15 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,46 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
The 15 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to rat Vesicle-associated membrane protein 2 (VAMP2).
Immunocytochemistry/ Immunofluorescence - VAMP2 antibody (ab82507)
ICC/IF image of ab82507 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82507, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
References for Anti-VAMP2 antibody (ab82507)
ab82507 has not yet been referenced specifically in any publications.
Publishing research using ab82507? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"