![Western blot - VAMP2 antibody [clone 3E5] (ab53407) image](#ab53407wb.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-VAMP2 antibody [clone 3E5]
See all VAMP2 products (9) ...
Description
Mouse monoclonal [clone 3E5] to VAMP2
Specificity
This antibody recognizes specifically VAMP2, but it also shows a low affinity to VAMP1.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Dog, Chimpanzee, Rhesus monkey
Immunogen
Recombinant fragment: MSATAATAPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV DKVLERDQKL SELDDRADAL QAGASQFETS AAKLKRKYW, corresponding to amino acids 1-89 of Human VAMP2
MSATAATAPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVD EVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETS AAKLKRKYW
Positive control
Rat brain extracts
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
clone 3E5
Myeloma
Sp2/0
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
-
Western blot - VAMP2 antibody [clone 3E5] (ab53407)
![Western blot - VAMP2 antibody [clone 3E5] (ab53407) image](/ps/datasheet/Images/53/ab53407/ab53407wb.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/3000. Predicted molecular weight: 13 kDa.
ELISA: Use at an assay dependent dilution.
Target
Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Tissue specificity
Nervous system and skeletal muscle.
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
Target information above from: UniProt accessionP63027
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
see all
Database links
- Entrez Gene: 6844 Human
- Omim: 185881 Human
- SwissProt: P63027 Human
- Unigene: 25348 Human
Anti-VAMP2 antibody [clone 3E5] images:
Western blot - VAMP2 antibody [clone 3E5] (ab53407)
All lanes : Anti-VAMP2 antibody [clone 3E5] (ab53407) at 1/2000 dilution
Lane 1 : Recombinant VAMP1
Lane 2 : Recombinant VAMP2
Lane 3 : Rat brain extracts
Lysates/proteins at 20 µg per lane.
Secondary
HRP-conjugated goat anti-mouse
developed using the ECL technique
Predicted band size : 13 kDa
Observed band size : 13,15,17 kDa (why is the actual band size different from the predicted?)
References for Anti-VAMP2 antibody [clone 3E5] (ab53407)
ab53407 has not yet been referenced specifically in any publications.
Publishing research using ab53407? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"