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Western blot - VAMP4 antibody (ab45061)
Western blot - VAMP4 antibody (ab45061)
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Product Name 

VAMP4 antibody - Vesicle Marker

 

See all VAMP4 antibodies (5)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to VAMP4 - Vesicle Marker

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human VAMP4.

(Peptide available as ab45264.)

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Cow
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

ab45061 gave a positive signal in the following Human Lysates: Brain, Testis, Liver, Hela and HepG2.

Cellular localization 

Golgi Apparatus

Research areas 

Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs

Relevance 

VAMP4 belongs to the synaptobrevin family and contains 1 v-SNARE coiled-coil homology domain. It is involved in the pathway that removes an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. Vamp4 may be a marker for this sorting pathway that is critical for remodeling the secretory response of the secretory granule. It may play a role in trans-Golgi network-to-endosome transport.

 

Alternative names VAMP4 antibody - Vesicle Marker (ab45061)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

616923    (Cow)
8674    (Human)
53330    (Mouse)

Omim

    

606909    (Human)

SwissProt

    

Q32L97    (Cow)
O75379    (Human)
A2IDD8    (Human)
Q6IAZ3    (Human)
O70480    (Mouse)

Unigene

    

6651    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.600 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VAMP4 antibody - Vesicle Marker is on this datasheet. But please do contact us if you would like any reassurance!


See below for VAMP4 antibody - Vesicle Marker images, references, products related to ab45061 and other tools.

 

VAMP4 antibody - Vesicle Marker images:

Product image  Western blot - VAMP4 antibody (ab45061)

Western blot - VAMP4 antibody (ab45061)

All lanes : VAMP4 antibody - Vesicle Marker (ab45061) at 1 µg/ml

Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 16 kDa
Observed band size : 16 kDa
Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.

 
 

Search PubMed (MEDLINE) for references to VAMP4

 

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VAMP4 antibody - Vesicle Marker - related products:

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Loading controls

Lysates

 

Immunizing Peptide for VAMP4 antibody - Vesicle Marker

ab45264

  

VAMP4 peptide

 

Compatible Secondaries for VAMP4 antibody - Vesicle Marker

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other VAMP4 Antibodies

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VAMP4 antibody - Vesicle Marker - other tools:

Contact Abcam with a Technical Enquiry about VAMP4 antibody - Vesicle Marker

 

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