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Products:Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
Overview
Product name
Anti-VAMP4 antibody - Vesicle Marker
See all VAMP4 products (7) ...
Description
Rabbit polyclonal to VAMP4 - Vesicle Marker
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human VAMP4.
(Peptide available as ab45264.)
Positive control
ab45061 gave a positive signal in the following Human Lysates: Brain, Testis, Liver, Hela and HepG2.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
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Western blot - VAMP4 antibody (ab45061)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
Target
Function
Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles.
Target information above from: UniProt accessionO75379
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- VAMP 24 antibody
- VAMP 4 antibody
- VAMP-4 antibody
see all
Database links
- Entrez Gene: 616923 Cow
- Entrez Gene: 8674 Human
- Entrez Gene: 53330 Mouse
- Omim: 606909 Human
- SwissProt: Q32L97 Cow
- SwissProt: A2IDD8 Human
- SwissProt: O75379 Human
- SwissProt: O70480 Mouse
see all
Anti-VAMP4 antibody - Vesicle Marker images:
Western blot - VAMP4 antibody (ab45061)
All lanes : Anti-VAMP4 antibody - Vesicle Marker (ab45061) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 16 kDa
Observed band size : 16 kDa
Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
References for Anti-VAMP4 antibody - Vesicle Marker (ab45061)
ab45061 has not yet been referenced specifically in any publications.
Publishing research using ab45061? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"