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ab45264 |
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Read our guarantee »Products:Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
Anti-VAMP4 antibody - Vesicle Marker
See all VAMP4 products (7) ...
Rabbit polyclonal to VAMP4 - Vesicle Marker
Reacts with
Human
Predicted to work with
Mouse, Cow
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human VAMP4.
(Peptide available as ab45264.)
ab45061 gave a positive signal in the following Human Lysates: Brain, Testis, Liver, Hela and HepG2.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
Western blot - VAMP4 antibody (ab45061)
(enlarge)
Our Abpromise guarantee covers the use of ab45061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Golgi apparatus > trans-Golgi network membrane. Associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles.
Target information above from: UniProt accessionO75379
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - VAMP4 antibody (ab45061)

All lanes : Anti-VAMP4 antibody - Vesicle Marker (ab45061) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 16 kDa
Observed band size : 16 kDa
Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
ab45061 has not yet been referenced specifically in any publications.
Publishing research using ab45061? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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All lanes : Anti-VAMP4 antibody - Vesicle Marker (ab45061) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 16 kDa
Observed band size : 16 kDa
Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
2
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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