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Anti-VAMP4 antibody (ab3348)

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Overview

Product name

Anti-VAMP4 antibody
See all VAMP4 products (7) ...

Description

Rabbit polyclonal to VAMP4

Tested applications

IF, WB, IHC-Fr, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Recombinant full length protein (Rat).

Positive control

WB: PC3 cell extract ICC: CV-1 cells

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS, 1 mg/ml BSA, 0.05% sodium azide

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Primary antibody notes

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP 25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, VAMP levels of all isoforms appear to be significantly lowered.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs

  • Western blot - VAMP4 antibody (ab3348)Western blot - VAMP4 antibody (ab3348) image (enlarge)

Applications

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Our Abpromise guarantee covers the use of ab3348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    IF

     IF: Use a concentration ...Read more →

    IF: Use a concentration of 10 µg/ml(Immunofluorescence staining with this antibody requires saponin permeabilization.)

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/mlDetects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).

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    IHC-Fr

     IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent dilution. (PubMed: 21116650)

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    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution. (PubMed: 17922004)

Target

Function

Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.

Sequence similarities

Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.

Cellular localization

Golgi apparatus > trans-Golgi network membrane. Associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles.

Target information above from: UniProt accessionO75379 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • VAMP 24 antibody
  • VAMP 4 antibody
  • VAMP-4 antibody
  • VAMP24 antibody
  • VAMP4 antibody
  • VAMP4 protein antibody
  • VAMP4_HUMAN antibody
  • Vesicle associated membrane protein 4 antibody
  • Vesicle associated membrane protein 4 isoform CRA a antibody
  • Vesicle associated membrane protein 4 isoform CRA b antibody
  • Vesicle-associated membrane protein 4 antibody
see all

Database links

see all

Anti-VAMP4 antibody images:

  Western blot - VAMP4 antibody (ab3348)

Western blot - VAMP4 antibody (ab3348)

Anti-VAMP4 antibody (ab3348) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 16 kDa
Observed band size : 16 kDa
Additional bands at : 36 kDa,49 kDa,75 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 30 seconds

References for Anti-VAMP4 antibody (ab3348)

This product has been referenced in:

  • Mehmood Tet al. Transcriptome profile reveals AMPA receptor dysfunction in the hippocampus of the Rsk2-knockout mice, an animal model of Coffin-Lowry syndrome. Hum Genet 129:255-69 (2011). WB, IHC-Fr; Mouse.Read more (PubMed: 21116650) »
  • Han Cet al. Human SCAMP5, a novel secretory carrier membrane protein, facilitates calcium-triggered cytokine secretion by interaction with SNARE machinery. J Immunol 182:2986-96 (2009). WB; Human, Mouse.Read more (PubMed: 19234194) »

See all 6 publications for this product

Publishing research using ab3348? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"