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All lanes : Anti-VAMP8 antibody [EP2629Y] (ab76021) at 1/20000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 11 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
ab76021, at a 1/100 dilution, staining VAMP8 in paraffin embedded human brain tissue by Immunohistochemistry. An HRP/AP polymerized secondary antibody was used.
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