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Anti-VAMP8 antibody [EP2629Y] (ab76021)

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Overview

Product name

Anti-VAMP8 antibody [EP2629Y]
See all VAMP8 products (4) ...

Description

Rabbit monoclonal [EP2629Y] to VAMP8

Tested applications

WB, IP, IHC-P, ICC, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues near the N terminal of human VAMP8

Positive control

293T, HeLa and PC12 cell lysates; human brain tissue

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP2629Y

Isotype

IgG

Research areas

Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs

  • Western blot - VAMP8 antibody [EP2629Y] (ab76021)Western blot - VAMP8 antibody [EP2629Y] (ab76021) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VAMP8 antibody [EP2629Y] (ab76021)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VAMP8 antibody [EP2629Y] (ab76021) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Flow Cyt: 1/80.
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
IP: 1/20.
WB: 1/10,000 - 1/20,000. Detects a band of approximately 17 kDa (predicted molecular weight: 11 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.

Tissue specificity

Platelets.

Sequence similarities

Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.

Cellular localization

Membrane.

Target information above from: UniProt accessionQ9BV40 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EDB antibody
  • Endobrevin antibody
  • VAMP 5 antibody
  • VAMP 8 antibody
  • VAMP-8 antibody
  • VAMP5 antibody
  • VAMP8 antibody
  • VAMP8_HUMAN antibody
  • Vesicle associated membrane protein 8 antibody
  • Vesicle-associated membrane protein 8 antibody
see all

Database links

see all

Anti-VAMP8 antibody [EP2629Y] images:

  Western blot - VAMP8 antibody [EP2629Y] (ab76021)

Western blot - VAMP8 antibody [EP2629Y] (ab76021)

All lanes : Anti-VAMP8 antibody [EP2629Y] (ab76021) at 1/20000 dilution

Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : PC12 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size : 11 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VAMP8 antibody [EP2629Y] (ab76021)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VAMP8 antibody [EP2629Y] (ab76021)

ab76021, at a 1/100 dilution, staining VAMP8 in paraffin embedded human brain tissue by Immunohistochemistry. An HRP/AP polymerized secondary antibody was used.

References for Anti-VAMP8 antibody [EP2629Y] (ab76021)

ab76021 has not yet been referenced specifically in any publications.

Publishing research using ab76021? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"