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regarding the vamp8 polyclonal antibody. could you provide more information regarding the human n terminal immunogen of vamp8 used? was the entire cytoplasmic domain of the endobrevin molecule used? ie what residues of human vamp8 specifically . thanks |
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ANSWER: |
Regarding the vamp8 polyclonal antibody, we could not give you the response of your questions because these informations are confidential. |
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I am interested in your VAMP3 and VANP8 antibody (Anti human) you don`t have reference for VAMP8. I wonder whether you can give me more information about this antibody |
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ANSWER: |
I agree that the information we have on the datasheet for ab6186 is minimal, but this is all I have to hand. If you let me know your specific questions, I can ask the originator of the antibody if they can help. The same goes for ab6185 -- Rabbit antiserum to human VAMP4 |
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What immunogen was used to produce this Ab? ie. what peptide sequence or site in the hVAMP8 sequence? |
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ANSWER: |
The immunogen for this antibody was a synthetic peptide derived from the N-terminal domain of human endobrevin/VAMP8. |
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What other species does this antibody cross-react with? |
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ANSWER: |
No cross-reactivity was found on rat PC12 and canine MDCK cell extracts. Cross reactivity in other species has not yet been tested. Specific reference: Lafont et al., PNAS 1999 |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-VAMP8 antibody (ab6186) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 11 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
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