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Anti-VAMP8 antibody (ab6186)

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Overview

Product name

Anti-VAMP8 antibody
See all VAMP8 products (4) ...

Description

Rabbit polyclonal to VAMP8

Specificity

Reacts specifically with the 10 kDa protein of human CaCo-2, rat PC12 and dog MDCK cell extracts.

Tested applications

WB, IP, IF, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Dog

Immunogen

N-terminal synthetic peptide (Human).

Positive control

human CaCo-2, rat PC12 and dog MDCK cell extracts. This antibody also gave a positive signal in the following Human whole cell lysates: A431; HEK293; HeLa.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole Serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs

  • Western blot - Anti-VAMP8 antibody (ab6186)Western blot - Anti-VAMP8 antibody (ab6186) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IF: 1/50.
IM: Use at an assay dependant dilution.
IP: Use at an assay dependant dilution.
WB: 1/200 - 1/1000.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.

Tissue specificity

Platelets.

Sequence similarities

Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.

Cellular localization

Membrane.

Target information above from: UniProt accessionQ9BV40 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EDB antibody
  • Endobrevin antibody
  • VAMP 8 antibody
  • VAMP-8 antibody
  • VAMP8 antibody
  • VAMP8_HUMAN antibody
  • Vesicle associated membrane protein 8 antibody
  • Vesicle-associated membrane protein 8 antibody
see all

Database links

Anti-VAMP8 antibody images:

  Western blot - Anti-VAMP8 antibody (ab6186)

Western blot - Anti-VAMP8 antibody (ab6186)

All lanes : Anti-VAMP8 antibody (ab6186) at 1 µg/ml

Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 11 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 2 minutes

References for Anti-VAMP8 antibody (ab6186)

This product has been referenced in:

  • Bethani Iet al. Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking. Traffic 10:1543-59 (2009). WB.Read more (PubMed: 19624487) »
  • Mashima Het al. Involvement of vesicle-associated membrane protein 7 in human gastric epithelial cell vacuolation induced by Helicobacter pylori-produced VacA. Infect Immun 76:2296-303 (2008).Read more (PubMed: 18362137) »

See all 6 publications for this product

Publishing research using ab6186? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"