VAMP8 antibody (ab6186)

Product Name 

VAMP8 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to VAMP8

Immunogen 

N-terminal synthetic peptide (Human).

Reacts with 

Hu

Predicted to react 
(species key)

Dog
(Due to sequence homology)

Specificity 

Reacts specifically with the 10 kDa protein of human CaCo-2, rat PC12 and dog MDCK cell extracts.

Tested applications 
(see key)

ICC/IF, IM, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IF: 1/50.
IM: Use at an assay dependant dilution.
IP: Use at an assay dependant dilution.
WB: 1/200 - 1/1000.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

human CaCo-2, rat PC12 and dog MDCK cell extracts

Cellular localization 

Membrane; Single pass type IV membrane protein

Research areas 

Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs

Relevance 

Synaptobrevins/VAMPs, syntaxins, and the 25kD synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF attachment protein (alpha SNAP), most likely through an VAMP8 containing SNARE complex. Also plays a role in regulated enzyme secretion in pancreatic acinar cells; the abscission of the midbody during cell division leading to completely separate daughter cells; and, the homotypic fusion of early and late endosomes.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole Serum

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for VAMP8 antibody (ab6186)

  This product has been used in: (two most recent references)

Bethani I et al. Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking. Traffic 10:1543-59 (2009). WB. PubMed: 19624487

Mashima H et al. Involvement of vesicle-associated membrane protein 7 in human gastric epithelial cell vacuolation induced by Helicobacter pylori-produced VacA. Infect Immun 76:2296-303 (2008). PubMed: 18362137

See all 4 publication references for this product.


If you publish research using ab6186 please let us know so that we can cite the reference on this datasheet.

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