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Western blot - VANGL2 antibody (ab60172)
(enlarge)
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Product Name
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VANGL2 antibody
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See all VANGL2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to VANGL2
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Immunogen
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Synthetic peptide: C-QKYLRTTKQQPYH, corresponding to internal sequence amino acids 403-415 of Human VANGL2
BLAST 'C-QKYLRTTKQQPYH' with  or 
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat, Xl, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: antibody detection limit dilution 1:32,000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse Brain lysate.
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Cellular localization
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Membrane; Multi-pass membrane protein
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Research areas
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Cardiovascular >> Heart >> Cardiogenesis >> Cardiomyocyte precursors
Stem Cells >> Signaling Pathways >> Wnt >> Surface Molecules
Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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VANGL2 is involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. It plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. It is required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VANGL2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VANGL2 antibody images, references, products related to ab60172 and other tools.
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VANGL2 antibody images:
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 Western blot - VANGL2 antibody (ab60172)
VANGL2 antibody (ab60172) at 0.3 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer)
Predicted band size : 60 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
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Search PubMed (MEDLINE) for references to VANGL2
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VANGL2 antibody - more information
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Customer reviews (feedback) regarding VANGL2 antibody |
Customer FAQs regarding VANGL2 antibody |
Protocols for VANGL2 antibody |
Price and availability of products related to VANGL2 antibody |
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VANGL2 antibody - related products:
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VANGL2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about VANGL2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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