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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-VANGL2 antibody
See all VANGL2 products (3) ...
Description
Goat polyclonal to VANGL2
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide: C-QKYLRTTKQQPYH, corresponding to internal sequence amino acids 403-415 of Human VANGL2
C-QKYLRTTK QQPYH
Positive control
Mouse Brain lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiogenesis >> Cardiomyocyte precursors
Stem Cells >> Signaling Pathways >> Wnt >> Surface Molecules
Neuroscience >> Neurology process >> Neurogenesis
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Western blot - VANGL2 antibody (ab60172)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab60172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: antibody detection limit dilution 1:32,000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process.
Sequence similarities
Belongs to the Vang family.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ9ULK5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Homolog of Drosophila strabismus antibody
- KIAA1215 antibody
- Loop tail associated protein antibody
see all
Database links
- Entrez Gene: 57216 Human
- Entrez Gene: 93840 Mouse
- Entrez Gene: 289229 Rat
- Entrez Gene: 245949 Zebrafish
- SwissProt: Q2TBC4 Human
- SwissProt: Q9ULK5 Human
- SwissProt: Q91ZD4 Mouse
- SwissProt: P84889 Rat
see all
Anti-VANGL2 antibody images:
Western blot - VANGL2 antibody (ab60172)
Anti-VANGL2 antibody (ab60172) at 0.3 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer)
Predicted band size : 60 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-VANGL2 antibody (ab60172)
ab60172 has not yet been referenced specifically in any publications.
Publishing research using ab60172? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"