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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-VANGL2 antibody
See all VANGL2 products (3) ...
Description
Rabbit polyclonal to VANGL2
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Rhesus monkey
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human VANGL2 .
(Peptide available as ab86632.)
Positive control
This antibody gave a positive signal in the following Whole Cell Lysates: HepG2; SHSY5Y; SKNBE; SKNSH
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiogenesis >> Cardiomyocyte precursors
Stem Cells >> Signaling Pathways >> Wnt >> Surface Molecules
Neuroscience >> Neurology process >> Neurogenesis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 10 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 54 kDa (predicted molecular weight: 60 kDa).
Target
Function
Involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process.
Sequence similarities
Belongs to the Vang family.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ9ULK5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Homolog of Drosophila strabismus antibody
- KIAA1215 antibody
- Loop tail associated protein antibody
see all
Database links
- Entrez Gene: 57216 Human
- Entrez Gene: 93840 Mouse
- Entrez Gene: 289229 Rat
- SwissProt: Q2TBC4 Human
- SwissProt: Q9ULK5 Human
- SwissProt: Q91ZD4 Mouse
- SwissProt: P84889 Rat
- Unigene: 99477 Human
see all
Anti-VANGL2 antibody images:
Western blot - VANGL2 antibody (ab76174)
All lanes : Anti-VANGL2 antibody (ab76174) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 4 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 60 kDa
Observed band size : 54 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Anti-VANGL2 antibody (ab76174)
ICC/IF image of ab76174 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76174, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-VANGL2 antibody (ab76174)
ab76174 has not yet been referenced specifically in any publications.
Publishing research using ab76174? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"