VAP-1 Human ELISA Kit (with color giving dyes) (ab119564)
Overview
- Product nameVAP-1 Human ELISA Kit (with color giving dyes)
- Precision
Intra-assay Sample n Mean SD CV% Overall 3% - Tests1 x 96 test
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity19 pg/ml
- Range31.3 pg/ml - 2000 pg/ml
- Recovery
Sample specific recovery Sample type Average % Range Serum 96 82% - 117% - Species reactivityReacts with: Human
- Product overview
ab119564 VAP-1 Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of Human sVAP-1. ab119564 is for research use only. Not for diagnostic or therapeutic procedures.
Lymphocyte binding to vascular endothelium is a prerequisite for the move- ment of immune cells from the blood into lymphoid tissues and into sites of inflammation. Under inflammatory conditions, cell surface expression of VAP-1 (vascular adhesion protein-1) which is an endothelial sialoglycoprotein, is induced. VAP-1 is a type II transmembrane protein of 84.6 kDa with a single transmembrane domain and N and O-glycosylation sites in the extracellular domain. In vivo, VAP-1 exists predominantly as a 180 kDa homodimer and functions both as an enzyme (monoamine oxidase) and an adhesion molecule for lymphocytes.
A circulating form of human Vascular Adhesion Protein-1 (sVAP-1) has been characterized. This sVAP-1 has been shown to be elevated in sera of patients with certain liver diseases and a correlation with the diagnosis of the patients was demonstrated.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Adhesive Films 4 units Assay Buffer Concentrate (20x) 1 x 5ml Biotin-Conjugate anti-human sVAP-1 monoclonal antibody 1 x 100µl Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml Human sVAP-1 Standard 2 vials Monoclonal anti-human sVAP-1-coated microwell plate 1 unit Red-Dye 1 x 0.4ml Stop Solution 1 x 15ml Streptavidin-HRP 1 x 150µl Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml -
Research Areas
modificationsTopaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
N- and O-glycosylated.
Target information above from: UniProt accession
Q16853
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- Amine oxidase copper containing 3AOC3AOC3_HUMAN
- Copper amine oxidaseHPAOMembrane primary amine oxidaseSemicarbazide-sensitive amine oxidaseSSAOVAP-1VAP1Vascular adhesion protein 1
see all
- Entrez Gene: 8639 Human
- Omim: 603735 Human
- SwissProt: Q16853 Human
- Unigene: 198241 Human
Applications
Our Abpromise guarantee covers the use of ab119564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
VAP-1 Human ELISA Kit (with color giving dyes) images
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Sandwich ELISA - VAP-1 Human ELISA Kit (with color giving dyes) (ab119564)Representative standard curve using ab119564.
Protocols
References for VAP-1 Human ELISA Kit (with color giving dyes) (ab119564)
ab119564 has not yet been referenced specifically in any publications.
