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Anti-VASH1 antibody (ab88435) at 1 µg/ml + Tagged recombinant Human VASH1 fragment (the immunogen) at 0.1 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 41 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.
Anti-VASH1 antibody (ab88435) at 1 µg/ml + HepG2 cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 41 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
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