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Products:Cardiovascular >> Angiogenesis >> Inhibitors
Overview
Product name
Anti-VASH1 antibody
See all VASH1 products (3) ...
Description
Mouse monoclonal to VASH1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: GGKKVAGGGS SGATPTSAAA TAPSGVRRLE TSEGTSAQRD EEPEEEGEED LRDGGVPFFV NRGGLPVDEA TWERMWKHVA KIHPDGEKVA QRIRGATD, corresponding to amino acids 3-100 of Human VASH1 (NP_055724) with a proprietary tag.
GGKKVAGGGS SGATPTSAAA TAPSGVRRLE TSEGTSAQRD EEPEEEGEED LRDGGVPFFV NRGGLPVDEA TWERMWKHVA KIHPDGEKVA QRIRGATD
Positive control
Tagged recombinant human VASH1 fragment (the immunogen). HepG2 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab88435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 41 kDa.
ELISA: Use at an assay dependent dilution.
Target
Function
Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity.
Tissue specificity
Preferentially expressed in endothelial cells. Highly expressed in fetal organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected in microvessels endothelial cells of atherosclerotic lesions.
Sequence similarities
Belongs to the vasohibin family.
Post-translational
modifications
2 major forms (42 and 36 kDa) and 2 minors (32 and 27 kDa) may be processed by proteolytic cleavage. The largest form (42 kDa) seems to be secreted and the other major form (63 kDa) seems to accumulate within the cells or pericellular milieu. Polypeptide consisting of Met-77 to Arg-318 may correspond to the 27 kDa form and that consisting of Met-77 to Val-365 may correspond to the 36 kDa form.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ7L8A9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- KIAA1036 antibody
- VASH antibody
- VASH1 antibody
see all
Database links
- Entrez Gene: 22846 Human
- Omim: 609011 Human
- SwissProt: Q7L8A9 Human
- Unigene: 525479 Human
Anti-VASH1 antibody images:
Western blot - VASH1 antibody (ab88435)
Anti-VASH1 antibody (ab88435) at 1 µg/ml + Tagged recombinant Human VASH1 fragment (the immunogen) at 0.1 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 41 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.
Western blot - VASH1 antibody (ab88435)
Anti-VASH1 antibody (ab88435) at 1 µg/ml + HepG2 cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 41 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
References for Anti-VASH1 antibody (ab88435)
ab88435 has not yet been referenced specifically in any publications.
Publishing research using ab88435? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"