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Western blot - VASH1 antibody (ab88435)
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Western blot - VASH1 antibody (ab88435)
(enlarge)
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Product Name
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VASH1 antibody
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See all VASH1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to VASH1
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Immunogen
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Recombinant fragment: GGKKVAGGGS SGATPTSAAA TAPSGVRRLE TSEGTSAQRD EEPEEEGEED LRDGGVPFFV NRGGLPVDEA TWERMWKHVA KIHPDGEKVA QRIRGATD, corresponding to amino acids 3-100 of Human VASH1 (NP_055724) with a proprietary tag.
BLAST 'GGKKVAGGGS SGATPTSAAA TAPSGVRRLE TSEGTSAQRD EEPEEEGEED LRDGGVPFFV NRGGLPVDEA TWERMWKHVA KIHPDGEKVA ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 41 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Tagged recombinant human VASH1 fragment (the immunogen). HepG2 cell lysate.
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Cellular localization
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Secreted
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Research areas
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Cardiovascular >> Angiogenesis >> Inhibitors
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Relevance
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VASH1 is a angiogenesis inhibitor. It inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. It does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis and acts in an autocrine manner. VASH1 also inhibits artery neointimal formation and macrophage infiltration and exhibits heparin-binding activity. There are two named isoforms.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VASH1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VASH1 antibody images, references, products related to ab88435 and other tools.
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VASH1 antibody images:
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 Western blot - VASH1 antibody (ab88435)
VASH1 antibody (ab88435) at 1 µg/ml + Tagged recombinant Human VASH1 fragment (the immunogen) at 0.1 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 41 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.
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Western blot - VASH1 antibody (ab88435)
VASH1 antibody (ab88435) at 1 µg/ml + HepG2 cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 41 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to VASH1
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VASH1 antibody - more information
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Customer reviews (feedback) regarding VASH1 antibody |
Customer FAQs regarding VASH1 antibody |
Protocols for VASH1 antibody |
Price and availability of products related to VASH1 antibody |
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VASH1 antibody - related products:
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VASH1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about VASH1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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