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Western blot - VASP antibody (ab26650)
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Western blot - VASP antibody (ab26650)
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Immunocytochemistry/ Immunofluorescence - VASP antibody (ab26650)
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Product Name
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VASP antibody
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See all VASP antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to VASP
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human VASP.
(Peptide available as ab32244.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 40 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line)
Jurkat (Human T cell lymphoblast-like cell line)
A431 (Human epithelial carcinoma cell line)
NIH 3T3 (Mouse embryonic fibroblast cell line)
MEF1 (Mouse embryonic fibroblast cell line)
PC12 (Rat adrenal pheochromocytoma cell line)
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Cellular localization
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Cytoplasmic; cytoskeleton, focal adhesions
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
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Relevance
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Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.270 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VASP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VASP antibody images, references, products related to ab26650 and other tools.
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VASP antibody images:
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 Immunocytochemistry/ Immunofluorescence - VASP antibody (ab26650)
ICC/IF image of ab26650 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab26650, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Search PubMed (MEDLINE) for references to VASP
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VASP antibody - more information
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Customer reviews (feedback) regarding VASP antibody |
Customer FAQs regarding VASP antibody |
Protocols for VASP antibody |
Price and availability of products related to VASP antibody |
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VASP antibody - related products:
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VASP antibody - other tools:
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Contact Abcam with a Technical Enquiry about VASP antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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