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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
Overview
Product name
Anti-VASP (phospho S239) antibody [16C2]
See all VASP products (14) ...
Description
Mouse monoclonal [16C2] to VASP (phospho S239)
Specificity
This antibody recognizes the ~46-50 kDa VASP protein phosphorylated at Ser239. It does not recognize the non-phosphorylated form of VASP.
Tested applications
WB, IP, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A synthetic phosphopeptide corresponding to amino acids surrounding the Ser239 phosphorylation site of human VASP, conjugated to KLH.
Positive control
Vanadate-treated A431 cells or 8-pCPT-treated platelets
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 2 x PBS, Polyethylene glycol, Sucrose
Concentration
Concentration information loading...
Purity
Size exclusion
Purification notes
Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography.
Clonality
Monoclonal
Clone number
16C2
Isotype
IgG1
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IP
ELISA
ICC/IF
Application notes
ELISA: Use at a concentration of 0.05 µg/ml.
ICC/IF: Use at a concentration of 1 - 10 µg/ml.
IP: Use 1-10µg for 106 cells.
WB: Use at a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa.
For immunocytochemistry, SNP or 8-pCPT-cGMP treated platelets are fixed with 4% paraformaldehyde in PBS for 15 minutes on ice, then permeabilized with 0.2% Triton® X-100 detergent in PBS for 10 min at room temperature.
For immunoblotting, block with 1% hemoglobin in PBS, 0.3% Triton® X-100 detergent, and 0.05% Tween®-20 detergent.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Tissue specificity
Highly expressed in platelets.
Sequence similarities
Belongs to the Ena/VASP family.
Contains 1 WH1 domain.
Domain
The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
The WH1 domain mediates interaction with XIRP1.
Post-translational
modifications
Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions.
Target information above from: UniProt accessionP50552
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Vasodilator stimulated phosphoprotein antibody
- Vasodilator-stimulated phosphoprotein antibody
- VASP antibody
- VASP_HUMAN antibody
Database links
- Entrez Gene: 7408 Human
- Entrez Gene: 22323 Mouse
- Omim: 601703 Human
- SwissProt: P50552 Human
- SwissProt: P70460 Mouse
- Unigene: 515469 Human
- Unigene: 9684 Mouse
References for Anti-VASP (phospho S239) antibody [16C2] (ab58554)
This product has been referenced in:
- Zuzga DSet al. Phosphorylation of vasodilator-stimulated phosphoprotein Ser239 suppresses filopodia and invadopodia in colon cancer. Int J Cancer : (2011).Read more (PubMed: 21702043) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"