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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Overview
Product name
Anti-VAV1 antibody
See all VAV1 products (6) ...
Description
Rabbit polyclonal to VAV1
Specificity
ab47403 detects endogenous levels of total VAV protein.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide, non-phosphopeptide derived from human VAV around the phosphorylation site of tyrosine 174.
Positive control
Jurkat cell extract.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
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Western blot - VAV1 antibody (ab47403)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 101 kDa.
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Function
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
Tissue specificity
Widely expressed in hematopoietic cells but not in other cell types.
Sequence similarities
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Domain
The DH domain is involved in interaction with CCPG1.
Post-translational
modifications
Phosphorylated on tyrosine residues.
Target information above from: UniProt accessionP15498
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Oncogene vav antibody
- p95Vav antibody
- Proto oncogene vav antibody
see all
Database links
- Entrez Gene: 7409 Human
- Entrez Gene: 22324 Mouse
- Entrez Gene: 25156 Rat
- Omim: 164875 Human
- SwissProt: P15498 Human
- SwissProt: P27870 Mouse
- SwissProt: P54100 Rat
- SwissProt: Q5BK91 Rat
see all
Anti-VAV1 antibody images:
Western blot - VAV1 antibody (ab47403)
All lanes : Anti-VAV1 antibody (ab47403) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with blocking peptide
Predicted band size : 101 kDa
Observed band size : 101 kDa
References for Anti-VAV1 antibody (ab47403)
ab47403 has not yet been referenced specifically in any publications.
Publishing research using ab47403? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"