Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
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ab4763 has been referenced in 3 publications.
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Predicted band size : 101 kDa
Peptide Competition: VAV 1 protein immunoprecipitated from K562 cell extracts was resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4763 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4763 blocks the antibody signal, thereby demonstrating the specificity of the antibody.
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