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Anti-VAV1 (phospho Y160) antibody (ab4763)

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1 question for ab4763

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Question 1

Friday 20-May-2005

The datasheet shows a Western blot using ab2722 after the protein is immunoprecipitated. Can this antibody be used on a lysate, instead of immunoprecipitated protein?

ANSWER:

  

Yes, this antibody can be used on a tissue lysate. I would recommend the following conditions: 1) Load 10-30 ug of the cell lysate, 2) Block the membrane with 5%BSA, 0.1% Tween in overnight at 4C, 3) Incubate the blocked blot with primary antibody at a concentration of 0.1-1.0 ug/mL in Tris buffered saline supplemented with 3% BSA and 0.1% Tween 20 for 2 hours at room temperature, 4) Wash the blot with several changes of Tris buffered saline supplemented with 0.1% Tween 20, 5) Detect the antibody band using an appropriate secondary antibody, in conjunction with your chemiluminescence reagents and instrumentation.

I hope this information helps you, please do not hesitate to contact me if you have further questions,

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