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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Overview
Product name
Anti-VAV1 (phospho Y160) antibody
See all VAV1 products (6) ...
Description
Rabbit polyclonal to VAV1 (phospho Y160)
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, a wide range of other species
Immunogen
Synthetic peptide (Human) derived from a region of VAV 1 that contains tyrosine 160. The sequence is conserved in mouse and rat.
Positive control
K562 cells expressing wild-type human Integrin alpha v and beta 3 proteins.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VAV 1. The final product is generated by affinity chromatography using a VAV 1 derived peptide that is phosphorylated at tyrosine 160.
Primary antibody notes
Vav proteins belong to the guanidine nucleotide exchange factor family of proteins that couple receptors to Rho-GTPases. The VAV family has currently three members in mammalian cells (VAV 1, VAV 2, and VAV 3). VAV 1 contains multiple protein binding motifs, indicating a role for VAV 1 as an adaptor protein linking cell surface receptors to downstream signaling proteins. VAV 1 is exclusively expressed in hematopoietic cells and plays a significant role in lymphocyte development and antigen receptor mediated activation of NFAT and NF(kappa)B, and in more delayed responses such as the production of cytokines, including IL-2, IL-3, IFN lambda, and TNF alpha. VAV 1 is also reported to activate JNK, p21-activated kinase (PAK) and PLC lambda, implicating its critical role in calcium mobilization, and cytoskeletal rearrangement. Activation of several cell surface receptors including integrins, immune response receptors, or growth factor receptors leads to phosphorylation of VAV 1 on tyrosine residues including tyrosine 160.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
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Western blot - VAV1 (phospho Y160) antibody (ab4763)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 21690326)
WB: Use a concentration of 0.1 - 1.0 µg/ml.Predicted molecular weight: 101 kDa.
Target
Function
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
Tissue specificity
Widely expressed in hematopoietic cells but not in other cell types.
Sequence similarities
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Domain
The DH domain is involved in interaction with CCPG1.
Post-translational
modifications
Phosphorylated on tyrosine residues.
Target information above from: UniProt accessionP15498
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Oncogene vav antibody
- p95Vav antibody
- Proto oncogene vav antibody
see all
Database links
- Entrez Gene: 7409 Human
- Entrez Gene: 22324 Mouse
- Entrez Gene: 25156 Rat
- Omim: 164875 Human
- SwissProt: P15498 Human
- SwissProt: P27870 Mouse
- SwissProt: P54100 Rat
- SwissProt: Q5BK91 Rat
see all
Anti-VAV1 (phospho Y160) antibody images:
Western blot - VAV1 (phospho Y160) antibody (ab4763)
Predicted band size : 101 kDa
Peptide Competition: VAV 1 protein immunoprecipitated from K562 cell extracts was resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4763 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4763 blocks the antibody signal, thereby demonstrating the specificity of the antibody.
References for Anti-VAV1 (phospho Y160) antibody (ab4763)
This product has been referenced in:
- Kaplan Aet al. Simulations of the NK Cell Immune Synapse Reveal that Activation Thresholds Can Be Established by Inhibitory Receptors Acting Locally. J Immunol 187:760-73 (2011). ICC/IF; Mouse.Read more (PubMed: 21690326) »
- Awasthi Aet al. Rap1b facilitates NK cell functions via IQGAP1-mediated signalosomes. J Exp Med 207:1923-38 (2010). WB; Mouse.Read more (PubMed: 20733035) »
See all 3 publications for this product
Publishing research using ab4763? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"