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Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)

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Overview

Product name

Anti-VAV1 (phospho Y174) antibody [EP510Y]
See all VAV1 products (6) ...

Description

Rabbit monoclonal [EP510Y] to VAV1 (phospho Y174)

Specificity

This antibody detects VAV1 phosphorylated at Tyrosine 174.

Tested applications

WB, ICCmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

Synthetic phophopeptide (Human) corresponding to residues surrounding Tyrosine 174

Positive control

Jurkat cell lysate treated with pervanadate

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP510Y

Isotype

IgG

Research areas

Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators

  • Western blot - VAV1 (phospho Y174) antibody [EP510Y] (ab76225)Western blot - VAV1 (phospho Y174) antibody [EP510Y] (ab76225) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/250000 - 1/500000....Read more →

    WB: 1/250000 - 1/500000.Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).

  • ICC

     ICC: 1/100 - 1/250.

    ICC: 1/100 - 1/250.

Application notes

Is unsuitable for ,Flow Cyt,IHC-P or IP.

Target

Function

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.

Tissue specificity

Widely expressed in hematopoietic cells but not in other cell types.

Sequence similarities

Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains.

Domain

The DH domain is involved in interaction with CCPG1.

Post-translational
modifications

Phosphorylated on tyrosine residues.

Target information above from: UniProt accessionP15498 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Oncogene vav antibody
  • p95Vav antibody
  • Proto oncogene vav antibody
  • Proto-oncogene vav antibody
  • Protooncogene vav antibody
  • VAV 1 antibody
  • VAV 1 oncogene antibody
  • VAV antibody
  • Vav proto oncogene antibody
  • VAV_HUMAN antibody
  • VAV1 antibody
see all

Database links

Anti-VAV1 (phospho Y174) antibody [EP510Y] images:

  Western blot - VAV1 (phospho Y174) antibody [EP510Y] (ab76225)

Western blot - VAV1 (phospho Y174) antibody [EP510Y] (ab76225)

All lanes : Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225) at 1/500000 dilution

Lane 1 : Untreated Jurkat cell lysate
Lane 2 : Jurkat cell lysate treated with pervanadate

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size : 101 kDa
Observed band size : 101 kDa

References for Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)

This product has been referenced in:

  • Liu HPet al. Association of supervillin with KIR2DL1 regulates the inhibitory signaling of natural killer cells. Cell Signal 23:487-96 (2011). WB; Human.Read more (PubMed: 21070852) »

See 1 publication for this product

Publishing research using ab76225? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"