VAV1 (phospho Y174) antibody [EP510Y] (ab76225)

Product Name 

VAV1 (phospho Y174) antibody [EP510Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP510Y] to VAV1 (phospho Y174)

Immunogen 

Synthetic phophopeptide (Human) corresponding to residues surrounding Tyrosine 174

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat

Specificity 

This antibody detects VAV1 phosphorylated at Tyrosine 174.

Tested applications 
(see key)

ICC, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: 1/100 - 1/250.


WB: 1/250000 - 1/500000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).

Is unsuitable for Flow Cyt, IHC-P or IP.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat cell lysate treated with pervanadate

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators

Relevance 

Vav proteins belong to the guanidine nucleotide exchange factor family of proteins that couple receptors to Rho-GTPases. The VAV family has currently three members in mammalian cells (VAV 1, VAV 2, and VAV 3). VAV 1 contains multiple protein binding motifs, indicating a role for VAV 1 as an adaptor protein linking cell surface receptors to downstream signaling proteins. VAV 1 is exclusively expressed in hematopoietic cells and plays a significant role in lymphocyte development and antigen receptor mediated activation of NFAT and NF(kappa)B, and in more delayed responses such as the production of cytokines, including IL-2, IL-3, IFN lambda, and TNF alpha. VAV 1 is also reported to activate JNK, p21-activated kinase (PAK) and PLC lambda, implicating its critical role in calcium mobilization, and cytoskeletal rearrangement. Activation of several cell surface receptors including integrins, immune response receptors, or growth factor receptors leads to phosphorylation of VAV 1 on tyrosine residues including tyrosine 160.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP510Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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