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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Anti-VAV2 antibody [EP1067Y] (ab52640)
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab52640 for help.
There are no answered questions for ab52640
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] (ab52640)](/ps/datasheet/images/52/ab52640/VAV2-Primary-antibodies-ab52640-3.jpg)
Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] (ab52640)
VAV2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Rabbit monoclonal to VAV2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52640.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 100kDa; VAV2.
![Western blot - VAV2 antibody [EP1067Y] (ab52640)](/ps/datasheet/Images/52/ab52640/vav1.jpg)
Western blot - VAV2 antibody [EP1067Y] (ab52640)
Anti-VAV2 antibody [EP1067Y] (ab52640) at 1/20000 dilution + 293 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 101 kDa
Observed band size : 101 kDa
![Immunohistochemistry (Paraffin-embedded sections) - VAV2 antibody [EP1067Y] (ab52640)](/ps/datasheet/Images/52/ab52640/vav2.jpg)
Immunohistochemistry (Paraffin-embedded sections) - VAV2 antibody [EP1067Y] (ab52640)
Human cervical carcinoma stained with ab52640 at 1/50 - 1/100 dilution
![VAV2 antibody [EP1067Y] for WB in Human (52640)](/ps/Reviews/Images/ab12269_68837.jpg)
VAV2 antibody [EP1067Y] for WB in Human (52640)
![VAV2 antibody [EP1067Y] for Western blot in Human (52640)](/ps/datasheet/images/52/ab52640/VAV2-Primary-antibodies-ab52640-1.jpg)
VAV2 antibody [EP1067Y] for Western blot in Human (52640)
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