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Anti-VAV2 antibody [EP1067Y] (ab52640)

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Overview

Product name

Anti-VAV2 antibody [EP1067Y]
See all VAV2 products (10) ...

Description

Rabbit monoclonal [EP1067Y] to VAV2

Specificity

This antibody detects Vav2 phosphorylated on Tyr172 as well as unphosphorylated Vav2.

Tested applications

WB, IP, ICC, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to residues on VAV2 (Human)

Positive control

Human cervical carcinoma 293 cell lysate

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP1067Y

Isotype

IgG

Research areas

Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other

  • Western blot - VAV2 antibody [EP1067Y] (ab52640)Western blot - VAV2 antibody [EP1067Y] (ab52640) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - VAV2 antibody [EP1067Y] (ab52640)Immunohistochemistry (Paraffin-embedded sections) - VAV2 antibody [EP1067Y] (ab52640) image (enlarge)

  • Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] (ab52640)Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] (ab52640) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab52640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for or Flow Cyt.

Target

Relevance

VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.

Cellular localization

Cytoplasmic

Database links

Anti-VAV2 antibody [EP1067Y] images:

  Western blot - VAV2 antibody [EP1067Y] (ab52640)

Western blot - VAV2 antibody [EP1067Y] (ab52640)

Anti-VAV2 antibody [EP1067Y] (ab52640) at 1/20000 dilution + 293 cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size : 101 kDa
Observed band size : 101 kDa

  Immunohistochemistry (Paraffin-embedded sections) - VAV2 antibody [EP1067Y] (ab52640)

Immunohistochemistry (Paraffin-embedded sections) - VAV2 antibody [EP1067Y] (ab52640)

Human cervical carcinoma stained with ab52640 at 1/50 - 1/100 dilution

  Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] (ab52640)

Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] (ab52640)

VAV2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10ug of Rabbit monoclonal to VAV2and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Hek293 whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52640. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Band: 100kDa; VAV2.

References for Anti-VAV2 antibody [EP1067Y] (ab52640)

This product has been referenced in:

  • Daubon Tet al. The Aarskog-Scott syndrome protein Fgd1 regulates podosome formation and extracellular matrix remodeling in transforming growth factor ß-stimulated aortic endothelial cells. Mol Cell Biol 31:4430-41 (2011).Read more (PubMed: 21911474) »
  • Kim HSet al. Synergistic signals for natural cytotoxicity are required to overcome inhibition by c-Cbl ubiquitin ligase. Immunity 32:175-86 (2010). WB; Human.Read more (PubMed: 20189481) »

See all 6 publications for this product

Publishing research using ab52640? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"