Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Overview
Product name
Anti-VAV2 antibody
See all VAV2 products (10) ...
Description
Goat polyclonal to VAV2
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide: ETEAKYYRTLEDIEC, corresponding to Internal sequence amino acids 208-221 of Human VAV2.
ETEAKYYRTL EDIEC
General notes
GenBank Accession Number – NP_003362.
LocusLink ID - 7410 (human); 22325 (mouse).
Gene Ontology terms - guanyl-nucleotide exchange factor activity; intracellular signaling cascade; diacylglycerol binding.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
-
Western blot - VAV2 antibody (ab4549)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
Peptide ELISA: antibody detection limit dilution >1/32000.
WB: Use at a concentration of 1 - 1.5 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.
Cellular localization
Cytoplasmic
Alternative names
- Oncogene VAV2 antibody
- Protein vav 2 antibody
- VAV 2 antibody
see all
Database links
- Entrez Gene: 7410 Human
- Entrez Gene: 22325 Mouse
- Omim: 600428 Human
- SwissProt: P52735 Human
- SwissProt: Q60992 Mouse
Anti-VAV2 antibody images:
Western blot - VAV2 antibody (ab4549)
Predicted band size : 100 kDa
ab4549 staining (1.5µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
References for Anti-VAV2 antibody (ab4549)
ab4549 has not yet been referenced specifically in any publications.
Publishing research using ab4549? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"