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Western blot - VAV2 antibody (ab4549)
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Product Name
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VAV2 antibody
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See all VAV2 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to VAV2
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Immunogen
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Synthetic peptide: ETEAKYYRTLEDIEC, corresponding to Internal sequence amino acids 208-221 of Human VAV2.
BLAST 'ETEAKYYRTLEDIEC' with  or 
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Reacts with
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Hu
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Predicted to react
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Ms
(Due to sequence homology)
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: antibody detection limit dilution >1/32000.
WB: Use at a concentration of 1 - 1.5 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
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Relevance
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VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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GenBank Accession Number – NP_003362.
LocusLink ID - 7410 (human); 22325 (mouse).
Gene Ontology terms - guanyl-nucleotide exchange factor activity; intracellular signaling cascade; diacylglycerol binding.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VAV2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VAV2 antibody images, references, products related to ab4549 and other tools.
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VAV2 antibody images:
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 Western blot - VAV2 antibody (ab4549)
Predicted band size : 100 kDa
ab4549 staining (1.5µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Search PubMed (MEDLINE) for references to VAV2
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VAV2 antibody - more information
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Customer reviews (feedback) regarding VAV2 antibody |
Customer FAQs regarding VAV2 antibody |
Protocols for VAV2 antibody |
Price and availability of products related to VAV2 antibody |
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VAV2 antibody - related products:
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VAV2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about VAV2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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