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Anti-VAV2 antibody (ab4549)

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Overview

Product name

Anti-VAV2 antibody
See all VAV2 products (10) ...

Description

Goat polyclonal to VAV2

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthetic peptide: ETEAKYYRTLEDIEC, corresponding to Internal sequence amino acids 208-221 of Human VAV2.

ETEAKYYRTL EDIEC

General notes

GenBank Accession Number – NP_003362. LocusLink ID - 7410 (human); 22325 (mouse). Gene Ontology terms - guanyl-nucleotide exchange factor activity; intracellular signaling cascade; diacylglycerol binding.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - VAV2 antibody (ab4549)Western blot - VAV2 antibody (ab4549) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab4549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Peptide ELISA: antibody detection limit dilution >1/32000.
WB: Use at a concentration of 1 - 1.5 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.

Cellular localization

Cytoplasmic

Alternative names

  • Oncogene VAV2 antibody
  • Protein vav 2 antibody
  • VAV 2 antibody
  • Vav 2 oncogene antibody
  • VAV2 oncogene antibody
see all

Anti-VAV2 antibody images:

  Western blot - VAV2 antibody (ab4549)

Western blot - VAV2 antibody (ab4549)



Predicted band size : 100 kDa


ab4549 staining (1.5µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References for Anti-VAV2 antibody (ab4549)

ab4549 has not yet been referenced specifically in any publications.

Publishing research using ab4549? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"