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Western blot - VAV2 antibody (ab86699)
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Immunocytochemistry/ Immunofluorescence - VAV2 antibody (ab86699)
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Product Name
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VAV2 antibody
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See all VAV2 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to VAV2
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Immunogen
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Synthetic peptide: EDEGDDIYED conjugated to KLH, corresponding to amino acids 165-174 of Mouse VAV2.
BLAST 'EDEGDDIYED' with  or 
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Reacts with
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Hu, Ms, Rb
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Predicted to react
(species key)
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Rat, Chk, Orngt
(Due to sequence homology)
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Specificity
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ab86699 detects VAV2 in human A431 cells and detects several VAV isoforms in Jurkat cells. The immunogen sequence has significant homology to the conserved site in VAV1 and VAV3.
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Tested applications
(see key)
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ELISA, ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/2000.
ICC/IF: 1/50.
IP: 1/100.
WB: 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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NP40 permeabilized rabbit spleen fibroblasts and A431 cells treated with EGF.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
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Relevance
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VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VAV2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VAV2 antibody images, references, products related to ab86699 and other tools.
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VAV2 antibody images:
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 Western blot - VAV2 antibody (ab86699)
All lanes : VAV2 antibody (ab86699) at 1/1000 dilution
Lane 1 : A431 cells treated with EGF
Lane 2 : A431 cells treated with EGF, exposed to alkaline
phosphatase
Predicted band size : 100 kDa
Observed band size : 100 kDa
Additional bands at : <100 kDa. We are unsure as to the identity of these extra bands.
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Immunocytochemistry/ Immunofluorescence - VAV2 antibody (ab86699)
Immunocytochemical labeling of VAV2 in paraformaldehyde-fixed and NP40 permeabilized rabbit spleen fibroblasts. The cells were labeled with ab86699 at a 1/50 dilution and detected using an appropriate secondary antibody conjugated to Cy3.
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Search PubMed (MEDLINE) for references to VAV2
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VAV2 antibody - more information
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Customer reviews (feedback) regarding VAV2 antibody |
Customer FAQs regarding VAV2 antibody |
Protocols for VAV2 antibody |
Price and availability of products related to VAV2 antibody |
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VAV2 antibody - related products:
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VAV2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about VAV2 antibody |
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