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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Overview
Product name
Anti-VAV2 antibody
See all VAV2 products (10) ...
Description
Rabbit polyclonal to VAV2
Specificity
ab86699 detects VAV2 in human A431 cells and detects several VAV isoforms in Jurkat cells. The immunogen sequence has significant homology to the conserved site in VAV1 and VAV3.
Tested applications
WB, IP, ICC/IF, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rabbit, Human
Predicted to work with
Rat, Chicken, Orangutan
Immunogen
Synthetic peptide: EDEGDDIYED conjugated to KLH, corresponding to amino acids 165-174 of Mouse VAV2.
EDEGDDIYED
Positive control
NP40 permeabilized rabbit spleen fibroblasts and A431 cells treated with EGF.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IP
IP: 1/100IP: 1/100
ICC/IF: 1/50
ELISA
ELISA: 1/2000ELISA: 1/2000
Target
Relevance
VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.
Cellular localization
Cytoplasmic
Database links
- Entrez Gene: 7410 Human
- Entrez Gene: 22325 Mouse
- Entrez Gene: 296603 Rat
- Omim: 600428 Human
- SwissProt: P52735 Human
- SwissProt: Q60992 Mouse
Anti-VAV2 antibody images:
Western blot - VAV2 antibody (ab86699)
All lanes : Anti-VAV2 antibody (ab86699) at 1/1000 dilution
Lane 1 : A431 cells treated with EGF
Lane 2 : A431 cells treated with EGF, exposed to alkaline
phosphatase
Predicted band size : 100 kDa
Observed band size : 100 kDa
Additional bands at : <100 kDa. We are unsure as to the identity of these extra bands.
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Immunocytochemistry/ Immunofluorescence - VAV2 antibody (ab86699)
Immunocytochemical labeling of VAV2 in paraformaldehyde-fixed and NP40 permeabilized rabbit spleen fibroblasts. The cells were labeled with ab86699 at a 1/50 dilution and detected using an appropriate secondary antibody conjugated to Cy3.
References for Anti-VAV2 antibody (ab86699)
ab86699 has not yet been referenced specifically in any publications.
Publishing research using ab86699? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"