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Western blot - VAV2 (phospho Y142) antibody (ab73707)
All lanes : Anti-VAV2 (phospho Y142) antibody (ab73707) at 1/500 dilution
Lane 1 : Extracts from K562 cells treated with TNF (200ng/ml, 30mins)
Lane 2 : Extracts from Hela cells treated with TNF (200ng/ml, 30mins)
Lane 3 : Extracts from K562 cells treated with TNF (200ng/ml, 30mins) plus 5µg immunizing phosphopeptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 101 kDa
Observed band size : 101 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VAV2 (phospho Y142) antibody (ab73707)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab73707 at a 1:50 dilution.
Left image un-treated.
Right image treated with immunizing phosphopeptide.
Immunocytochemistry/ Immunofluorescence - VAV2 (phospho Y142) antibody (ab73707)
Immunofluorescence analysis of NIH/3T3 cells using ab73707 at a 1:500 dilution.
Left image un-treated.
Right image treated with immunizing phosphopeptide.
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