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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Overview
Product name
Anti-VAV2 (phospho Y142) antibody
See all VAV2 products (10) ...
Description
Rabbit polyclonal to VAV2 (phospho Y142)
Specificity
This antibody detects endogenous levels of VAV2 only when phosphorylated at tyrosine 142.
Tested applications
WB, ELISA, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
A synthesized phosphopeptide derived from human VAV2 around the phosphorylation site of tyrosine 142 (D-V-YP-R-S).
Positive control
Human brain tissue sections, NIH/3T3 cells and extracts from K562 cells and Hela cells, treated with TNF.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab73707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/50 - 1/100.
ICC/IF: 1/500 - 1/1000.
Target
Relevance
VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.
Cellular localization
Cytoplasmic
Alternative names
- Oncogene VAV2 antibody
- Protein vav 2 antibody
- VAV 2 antibody
see all
Database links
- Entrez Gene: 7410 Human
- Entrez Gene: 22325 Mouse
- Omim: 600428 Human
- SwissProt: P52735 Human
- SwissProt: Q60992 Mouse
Anti-VAV2 (phospho Y142) antibody images:
Western blot - VAV2 (phospho Y142) antibody (ab73707)
All lanes : Anti-VAV2 (phospho Y142) antibody (ab73707) at 1/500 dilution
Lane 1 : Extracts from K562 cells treated with TNF (200ng/ml, 30mins)
Lane 2 : Extracts from Hela cells treated with TNF (200ng/ml, 30mins)
Lane 3 : Extracts from K562 cells treated with TNF (200ng/ml, 30mins) plus 5µg immunizing phosphopeptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 101 kDa
Observed band size : 101 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VAV2 (phospho Y142) antibody (ab73707)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab73707 at a 1:50 dilution.
Left image un-treated.
Right image treated with immunizing phosphopeptide.
Immunocytochemistry/ Immunofluorescence - VAV2 (phospho Y142) antibody (ab73707)
Immunofluorescence analysis of NIH/3T3 cells using ab73707 at a 1:500 dilution.
Left image un-treated.
Right image treated with immunizing phosphopeptide.
References for Anti-VAV2 (phospho Y142) antibody (ab73707)
ab73707 has not yet been referenced specifically in any publications.
Publishing research using ab73707? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"