VAV2 (phospho Y172) antibody (ab86695)

Product Name 

VAV2 (phospho Y172) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to VAV2 (phospho Y172)

Immunogen 

Synthetic peptide conjugated to KLH corresponding to amino acid residues surrounding Tyr-172 in human VAV2. This sequence has high homology with similar regions in rat and mouse VAV2, and has significant homology to the conserved site in Vav1 and Vav3.

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat
(Due to sequence homology)

Specificity 

ab86695 detects Vav1, Vav2, and Vav3 in Jurkat cells treated with pervanadate. The antibody does not detect these Vavs after treatment with alkaline phosphatase.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/2000.
WB: 1/1000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A431 cells treated with EGF.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other

Relevance 

VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab86695 was cross-adsorbed to unphosphorylated VAV2 (Tyr-172) peptide before affinity purification using phospho-VAV2 (Tyr-172) peptide (without carrier).

Form 

Liquid

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

See below for VAV2 (phospho Y172) antibody images, references, products related to ab86695 and other tools.

VAV2 (phospho Y172) antibody images:

Customer reviews (feedback) regarding VAV2 (phospho Y172) antibody

Customer FAQs regarding VAV2 (phospho Y172) antibody

Protocols for VAV2 (phospho Y172) antibody

Price and availability of products related to VAV2 (phospho Y172) antibody

VAV2 (phospho Y172) antibody - related products: