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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Overview
Product name
Anti-VAV2 (phospho Y172) antibody
See all VAV2 products (10) ...
Description
Rabbit polyclonal to VAV2 (phospho Y172)
Specificity
ab86695 detects Vav1, Vav2, and Vav3 in Jurkat cells treated with pervanadate. The antibody does not detect these Vavs after treatment with alkaline phosphatase.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide conjugated to KLH corresponding to amino acid residues surrounding Tyr-172 in human VAV2. This sequence has high homology with similar regions in rat and mouse VAV2, and has significant homology to the conserved site in Vav1 and Vav3.
Positive control
A431 cells treated with EGF.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab86695 was cross-adsorbed to unphosphorylated VAV2 (Tyr-172) peptide before affinity purification using phospho-VAV2 (Tyr-172) peptide (without carrier).
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
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Western blot - VAV2 (phospho Y172) antibody (ab86695)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
ELISA
ELISA: 1/2000ELISA: 1/2000
Target
Relevance
VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.
Cellular localization
Cytoplasmic
Alternative names
- Oncogene VAV2 antibody
- Protein vav 2 antibody
- VAV 2 antibody
see all
Database links
- Entrez Gene: 7410 Human
- Entrez Gene: 22325 Mouse
- Entrez Gene: 296603 Rat
- Omim: 600428 Human
- SwissProt: P52735 Human
- SwissProt: Q60992 Mouse
Anti-VAV2 (phospho Y172) antibody images:
Western blot - VAV2 (phospho Y172) antibody (ab86695)
All lanes : Anti-VAV2 (phospho Y172) antibody (ab86695) at 1/1000 dilution
Lane 1 : A431 cells treated with EGF
Lane 2 : A431 cells treated with EGF, exposed to alkaline phosphatase
Predicted band size : 101 kDa
Observed band size : 101 kDa
Additional bands at : <100 kDa. We are unsure as to the identity of these extra bands.
References for Anti-VAV2 (phospho Y172) antibody (ab86695)
ab86695 has not yet been referenced specifically in any publications.
Publishing research using ab86695? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"