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Read our guarantee »Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Anti-VAV3 antibody [EP1130Y]
See all VAV3 products (4) ...
Rabbit monoclonal [EP1130Y] to VAV3
WB, IP, Flow Cytmore details
Reacts with
Mouse, Human
Does not react with
Rat
A synthetic peptide corresponding to residues near the C-term of human Vav3.
Jurkat cell lysate; permeabalized Jurkat cells.
Liquid
Store at -20°C. Stable for 12 months at -20°C
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Tissue culture supernatant
Monoclonal
EP1130Y
IgG
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Cancer >> Cell cycle >> Cell division
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Our Abpromise guarantee covers the use of ab52938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100000Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IP: 1/50
Flow Cyt: 1/40
Is unsuitable for or IHC-P.
Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Target information above from: UniProt accessionQ9UKW4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - VAV3 antibody [EP1130Y] (ab52938)
![Western blot - VAV3 antibody [EP1130Y] (ab52938)](/ps/datasheet/Images/52/ab52938/ab52938wb.jpg)
Anti-VAV3 antibody [EP1130Y] (ab52938) at 1/100000 dilution + Jurkat cell lysate at 10 µg
Secondary
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 98 kDa
Observed band size : ~98 kDa (why is the actual band size different from the predicted?)
Flow Cytometry - VAV3 antibody [EP1130Y] (ab52938)
![Flow Cytometry - VAV3 antibody [EP1130Y] (ab52938)](/ps/datasheet/Images/52/ab52938/ab52938fc.jpg)
Flow cytometric analysis of permeabilized Jurkat cells using ab52938 (red) or a rabbit IgG (negative) (green).
ab52938 has not yet been referenced specifically in any publications.
Publishing research using ab52938? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
![Western blot - VAV3 antibody [EP1130Y] (ab52938)](/ps/datasheet/Images/52/ab52938/ab52938wb.jpg)
Anti-VAV3 antibody [EP1130Y] (ab52938) at 1/100000 dilution + Jurkat cell lysate at 10 µg
Secondary
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 98 kDa
Observed band size : ~98 kDa (why is the actual band size different from the predicted?)
![Flow Cytometry - VAV3 antibody [EP1130Y] (ab52938)](/ps/datasheet/Images/52/ab52938/ab52938fc.jpg)
Flow cytometric analysis of permeabilized Jurkat cells using ab52938 (red) or a rabbit IgG (negative) (green).
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