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Western blot - VAV3 antibody [EP1130Y] (ab52938)
![Western blot - VAV3 antibody [EP1130Y] (ab52938)](/ps/datasheet/images/ab52938wb.jpg)
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Flow Cytometry - VAV3 antibody [EP1130Y] (ab52938)
![Flow Cytometry - VAV3 antibody [EP1130Y] (ab52938)](/ps/datasheet/images/ab52938fc.jpg)
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Product Name
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VAV3 antibody [EP1130Y]
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See all VAV3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EP1130Y] to VAV3
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Immunogen
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A synthetic peptide corresponding to residues near the C-term of human Vav3.
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Reacts with
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Hu, Ms
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Does not react with
(species key)
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Rat
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Tested applications
(see key)
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Flow Cyt, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/40.
IP: 1/50.
WB: 1/100000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
Is unsuitable for IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate; permeabalized Jurkat cells.
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Cellular localization
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Cell Membrane and Cytoplasmic
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Research areas
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Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Cancer >> Cell cycle >> Cell division
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
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Relevance
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Vav proteins are members of the Dbl-family of GEFs (Guanine Nucleotide Exchange Factors) for the Rho/Rac-family GTPases which activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. Vav proteins play pivotal roles in cytoskeletal re-organization in processes such as cell division and adhesion, phagocytosis, and platelet activation.
At least three Vav isoforms have been identified; Vav1, Vav2 and Vav3.
VAV3 is expressed in many tissues and participates in signal transduction processes. It is involved in the activation of several members of the Rho family. This activity, when deregulated, leads to marked cytoskeletal changes and to alterations in the process of cell division. Vav3 promotes PLC and PI3 Kinase activation, thus mediating Ca2+-dependent signalling.
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VAV3 antibody [EP1130Y] (ab52938) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EP1130Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VAV3 antibody [EP1130Y] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VAV3 antibody [EP1130Y] images, references, products related to ab52938 and other tools.
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VAV3 antibody [EP1130Y] images:
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 Western blot - VAV3 antibody [EP1130Y] (ab52938)
VAV3 antibody [EP1130Y] (ab52938) at 1/100000 dilution + Jurkat cell lysate at 10 µg
Secondary
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 98 kDa
Observed band size : ~98 kDa (why is the actual band size different from the predicted?)
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Flow Cytometry - VAV3 antibody [EP1130Y] (ab52938)
Flow cytometric analysis of permeabilized Jurkat cells using ab52938 (red) or a rabbit IgG (negative) (green).
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Search PubMed (MEDLINE) for references to VAV3
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VAV3 antibody [EP1130Y] - more information
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Customer reviews (feedback) regarding VAV3 antibody [EP1130Y] |
Customer FAQs regarding VAV3 antibody [EP1130Y] |
Protocols for VAV3 antibody [EP1130Y] |
Price and availability of products related to VAV3 antibody [EP1130Y] |
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VAV3 antibody [EP1130Y] - related products:
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VAV3 antibody [EP1130Y] - other tools:
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Contact Abcam with a Technical Enquiry about VAV3 antibody [EP1130Y] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - VAV3 antibody [EP1130Y] (ab52938)](/index.html?datasheet=52938#ab52938wb.jpg)
![Flow Cytometry - VAV3 antibody [EP1130Y] (ab52938)](/index.html?datasheet=52938#ab52938fc.jpg)
