![Western blot - VAV3 antibody [EP1130Y] (ab52938) image](#ab52938wb.jpg)
![Flow Cytometry - VAV3 antibody [EP1130Y] (ab52938) image](#ab52938fc.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Overview
Product name
Anti-VAV3 antibody [EP1130Y]
See all VAV3 products (4) ...
Description
Rabbit monoclonal [EP1130Y] to VAV3
Tested applications
WB, IP, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Does not react with
Rat
Immunogen
A synthetic peptide corresponding to residues near the C-term of human Vav3.
Positive control
Jurkat cell lysate; permeabalized Jurkat cells.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1130Y
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Cancer >> Cell cycle >> Cell division
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100000Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IP
IP: 1/50IP: 1/50
Flow Cyt: 1/40
Application notes
Is unsuitable for or IHC-P.
Target
Function
Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
Sequence similarities
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Target information above from: UniProt accessionQ9UKW4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ40431 antibody
- Guanine nucleotide exchange factor VAV3 antibody
- Protein vav 3 antibody
see all
Database links
- Entrez Gene: 10451 Human
- Entrez Gene: 57257 Mouse
- Omim: 605541 Human
- SwissProt: Q9UKW4 Human
- SwissProt: Q9R0C8 Mouse
- Unigene: 267659 Human
- Unigene: 103805 Mouse
- Unigene: 282257 Mouse
Anti-VAV3 antibody [EP1130Y] images:
Western blot - VAV3 antibody [EP1130Y] (ab52938)
![Western blot - VAV3 antibody [EP1130Y] (ab52938)](/ps/datasheet/Images/52/ab52938/ab52938wb.jpg)
Anti-VAV3 antibody [EP1130Y] (ab52938) at 1/100000 dilution + Jurkat cell lysate at 10 µg
Secondary
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 98 kDa
Observed band size : ~98 kDa (why is the actual band size different from the predicted?)
Flow Cytometry - VAV3 antibody [EP1130Y] (ab52938)
![Flow Cytometry - VAV3 antibody [EP1130Y] (ab52938)](/ps/datasheet/Images/52/ab52938/ab52938fc.jpg)
Flow cytometric analysis of permeabilized Jurkat cells using ab52938 (red) or a rabbit IgG (negative) (green).
References for Anti-VAV3 antibody [EP1130Y] (ab52938)
ab52938 has not yet been referenced specifically in any publications.
Publishing research using ab52938? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"