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If your product does not perform as described on this datasheet, we will refund or replace your product...
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LOT NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal or weak signal. Trying to detect VAV3 in glioma stem cells. Lots of background but no bad in 90kD expected size. Also ran 30ug of Jurkate cell lysate as positive control (ab14842) and there was no signal in that lane at all. SAMPLE protein lysates from glioma stem cells and jurkat cell lysate (ab14842) as control PRIMARY ANTIBODY ab14842, 1:500 4C overnight incubation DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED jurkat cell lysate (ab14842) ANTIBODY STORAGE CONDITIONS -20C SAMPLE PREPARATION Protein obtained by lysing cells in RIPA buffer with Protease inhibitors prepared in bond breaker, ripa buffer, 4x load buffer 95C for 10 minutes prior to loading AMOUNT OF PROTEIN LOADED 30ug TRANSFER AND BLOCKING CONDITIONS 5% milk in TBST 1 hour in RT SECONDARY ANTIBODY invitrogen rabbit anti-goat (81-1620) 1:2000, stored at 4C incubate in 5% Milk TBST 1 hours room temp HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes |
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ANSWER: |
Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed your filled-in questionnaire, I think your protocol looks fine. Could you please confirm a couple further details: 1) What species are your samples from? 2) Is the secondary antibody working well with other primary antibodies? Please let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. In that case, please let me know how you would like to proceed. I look forward to hear back from you and assist you in resolving this case. |
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