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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Overview
Product name
Anti-VAV3 antibody
See all VAV3 products (4) ...
Description
Goat polyclonal to VAV3
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Positive control
Human Bone Marrow lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Cancer >> Cell cycle >> Cell division
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
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Western blot - VAV3 antibody (ab21208)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 19546854)
WB: Use a concentration of 1 - 3 µg/ml.Predicted molecular weight: 90 kDa.
IHC-P
IHC-P: 1/200IHC-P: 1/200
Target
Function
Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
Sequence similarities
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Target information above from: UniProt accessionQ9UKW4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ40431 antibody
- Guanine nucleotide exchange factor VAV3 antibody
- Protein vav 3 antibody
see all
Database links
- Entrez Gene: 10451 Human
- Omim: 605541 Human
- SwissProt: Q9UKW4 Human
- Unigene: 267659 Human
Anti-VAV3 antibody images:
Western blot - VAV3 antibody (ab21208)
Anti-VAV3 antibody (ab21208) at 1 µg/ml + Human Bone Marrow lysate (35µg protein in RIPA buffer).
Predicted band size : 90 kDa
Observed band size : 98 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-VAV3 antibody (ab21208)
This product has been referenced in:
- Blair HCet al. Osteopetrosis with micro-lacunar resorption because of defective integrin organization. Lab Invest 89:1007-17 (2009). WB, ICC/IF; Human.Read more (PubMed: 19546854) »
- Salhia Bet al. The guanine nucleotide exchange factors trio, Ect2, and Vav3 mediate the invasive behavior of glioblastoma. Am J Pathol 173:1828-38 (2008). IHC-P; Human.Read more (PubMed: 19008376) »
See all 2 publications for this product
Publishing research using ab21208? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"