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Western blot - VAV3 antibody (ab40889)
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Immunohistochemistry (Paraffin-embedded sections) - VAV3 antibody (ab40889)
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Product Name
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VAV3 antibody
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See all VAV3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to VAV3
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Immunogen
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Synthetic peptide corresponding to residues near the C-term of human Vav3.
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Reacts with
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Hu, Ms
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Does not react with
(species key)
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Rat
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/50 - 1/100.
ICC/IF: 1/50 - 1/100.
IHC-P: 1/50 - 1/100.
IP: 1/50.
WB: 1/500. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurket cell lysate, human breast carcinoma.
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Cellular localization
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Cell Membrane and Cytoplasmic
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Research areas
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Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Cancer >> Cell cycle >> Cell division
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
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Relevance
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Vav proteins are members of the Dbl-family of GEFs (Guanine Nucleotide Exchange Factors) for the Rho/Rac-family GTPases which activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. Vav proteins play pivotal roles in cytoskeletal re-organization in processes such as cell division and adhesion, phagocytosis, and platelet activation.
At least three Vav isoforms have been identified; Vav1, Vav2 and Vav3.
VAV3 is expressed in many tissues and participates in signal transduction processes. It is involved in the activation of several members of the Rho family. This activity, when deregulated, leads to marked cytoskeletal changes and to alterations in the process of cell division. Vav3 promotes PLC and PI3 Kinase activation, thus mediating Ca2+-dependent signalling.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VAV3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VAV3 antibody images, references, products related to ab40889 and other tools.
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VAV3 antibody images:
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 Western blot - VAV3 antibody (ab40889)
VAV3 antibody (ab40889) at 1/500 dilution + Jurket cell lysate at 10 µg
Predicted band size : 95 kDa
Observed band size : 95 kDa
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Immunohistochemistry (Paraffin-embedded sections) - VAV3 antibody (ab40889)
Staining of Vav3 on paraffin-embedded human breast carcinom, using ab40889 at a 1:50 dilution.
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Search PubMed (MEDLINE) for references to VAV3
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VAV3 antibody - more information
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Customer reviews (feedback) regarding VAV3 antibody |
Customer FAQs regarding VAV3 antibody |
Protocols for VAV3 antibody |
Price and availability of products related to VAV3 antibody |
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VAV3 antibody - related products:
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VAV3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about VAV3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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