VCAM1 Human ELISA Kit (ab100661)
- Product nameVCAM1 Human ELISA KitSee all VCAM1 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 0.3 ng/ml
- Range0.247 ng/ml - 60 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.76 85% - 104% Serum 94.42 83% - 103% Plasma 93.58 82% - 102%
- Species reactivityReacts with: Human
- Product overview
ab100661 Human VCAM1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VCAM1 in serum, plasma, cell culture supernatants. This assay employs an antibody specific for Human VCAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and VCAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VCAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VCAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A animal serum . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody VCAM1 : biotinylated anti-human VCAM1 2 vials HRP-Streptavidin Concentrate 320x concentrated HRP-conjugated streptavidin 1 x 200µl Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml VCAM 1 Microplate (12 strips x 8 wells) coated with anti-human VCAM-1 1 unit VCAM 1 Standard Human(recombinant) 2 vials
- CD106 CD106 antigenDKFZp779G2333
- INCAM 100INCAM100L1CAM MGC99561 V CAM 1Vascular cell adhesion molecule 1Vascular cell adhesion protein 1VCAM 1
Our Abpromise guarantee covers the use of ab100661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for VCAM1 Human ELISA Kit (ab100661)
ab100661 has not yet been referenced specifically in any publications.