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Overview

  • Product nameVCAM1 Human ELISA KitSee all VCAM1 kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 8 48.79ng/ml 0.221 0.45%
    B 8 5.81ng/ml 0.132 2.27%
    Inter-assay
    Sample n Mean SD CV%
    A 28 49.69ng/ml 0.72 1.44%
    B 28 5.95ng/ml 0.354 5.94%
  • Tests
    2 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 0.6 ng/ml
  • Range
    1.56 ng/ml - 50 ng/ml
  • Assay time
    1h 40m
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human VCAM1 ELISA is to be used for the in-vitro quantitative determination of soluble Vascular Cellular Adhesion Molecule -1 (VCAM1) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human VCAM1. This kit has been configured for research use only and is not to be used in diagnostic procedures.

    Principal:

    The VCAM1 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for VCAM1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known VCAM1 concentrations, control specimens and unknowns are pipetted into these wells. During the first incubation, the VCAM1 antigen and a biotinylated monoclonal antibody specific for VCAM1 are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of VCAM1 present in the samples.

     

    Recognizes both natural and recombinant human VCAM1

    Range: 1.56 ng/ml - 50 ng/ml

    Sensitivity <0.6 ng/ml

  • Notes

    Safety Notes:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • RelevanceVCAM1 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
  • Cellular localizationCell Membrane; single-pass type I membrane protein
    • Alternative names
        CD106 CD106 antigenDKFZp779G2333
        INCAM 100INCAM100L1CAM MGC99561 V CAM 1Vascular cell adhesion molecule 1Vascular cell adhesion protein 1VCAM 1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab46118 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      VCAM1 Human ELISA Kit images

      • ELISA - Human VCAM1 ELISA Kit (ab46118)
        ELISA - Human VCAM1 ELISA Kit (ab46118)
        Typical VCAM1 standard curve ranging from 1.56 to 50 ng/mL.

      References for VCAM1 Human ELISA Kit (ab46118)

      ab46118 has not yet been referenced specifically in any publications.

      Publishing research using ab46118 ? Please let us know so that we can cite the reference in this datasheet

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      What formula do you recommend for creating the standard curve?

      Thank you for contacting us. The laboratory that developed and validated the kit uses linear regression but there are some who argue that 4 parameter logisitic nonlinear regression is a better way to curve-fit ELISA data. Either formula is applicable ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"