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Full length protein (Human).
Our Abpromise guarantee covers the use of ab7224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab7224 staining VCAM1 in Human pluripotent stem cell derived cardiomyocytes by Flow Cytometry. Cells were cultured in a monolayer with PBS + 2% FBS. The sample was incubated with the primary antibody (1/100 in PBS and 2% FBS) for 30 minutes at 4°C. A Biotin-conjugated mouse IgG (1/1000) was used as the secondary antibody.
Gating Strategy: Negative control population of pluripotent stem cells.
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