Overview

  • Product nameAnti-VCAM1 antibody [1G11B1] (Biotin)See all VCAM1 primary antibodies ...
  • Description
    Mouse monoclonal [1G11B1] to VCAM1 (Biotin)
  • ConjugationBiotin
  • Conjugation notesBiotin
  • Tested applicationsICC, IP, IHC-Fr, Flow Cyt more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human).

  • EpitopeEpitope is unknown. This clone is also known as 1.G11B1 and 1G11.B1
  • Positive control
    • Lymph nodes can be used as positive control. As negative control it is advised to use as first antibody a control murine IgG1 antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab7224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC
IP
IHC-Fr
Flow Cyt
  • Application notesThe antibody is extremely useful for staining of VCAM-1 expressing endothelial cells.
    It permits staining of in vitro cultured cells and frozen tissue sections.
    The antibody can be used for flow cytometry and immunoprecipitation.

    In vitro cultured cells can be fixed with 1% paraformaldehyde. Tissue sections should be fixed for 10 min in pure acetone followed by incubation for 10 min in chloroform.
    Incubation with a pretested dilution of the antibody is advised followed by a biotin-conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin.

    For selection of the most useful dilution in a given situation, a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 8 hours with TNF alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 ug/ml) in tissue culture medium at 37°C.
  • Target

    • FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
    • Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
    • Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
    • DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
    • Post-translational
      modifications
      Sialoglycoprotein.
    • Cellular localizationMembrane.
    • Target information above from: UniProt accession P19320 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CD 106 antibody
      • CD106 antibody
      • CD106 Antigen antibody
      • DKFZp779G2333 antibody
      • INCAM 100 antibody
      • INCAM-100 antibody
      • L1CAM antibody
      • MGC99561 antibody
      • V-CAM 1 antibody
      • Vascular Cell Adhesion Molecule 1 antibody
      • Vascular cell adhesion protein 1 antibody
      • VCAM 1 antibody
      • VCAM-1 antibody
      • VCAM1 antibody
      • VCAM1_HUMAN antibody
      see all

    References for Anti-VCAM1 antibody [1G11B1] (Biotin) (ab7224)

    This product has been referenced in:
    • Elliott DA  et al. NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes. Nat Methods 8:1037-40 (2011). Flow Cyt ; Human . Read more (PubMed: 22020065) »

    See 1 Publication for this product

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    Thank you for your enquiry. I am sorry you had trouble getting through to us. This antibody has not yet been tested in ELISA< so I cannot give you a recommended dilution. However, s general recommendation of a starting dilution for a purified anti...

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