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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
Full length protein (Human).
Our Abpromise guarantee covers the use of ab7219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.
The antibody is extremely useful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. The antibody can be used for flow cytometry and immunoprecipitation. In vitro cultured cells can be fixed with 1% paraformaldehyde. Tissue sections should be fixed for 10 min in pure acetone followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised followed by a biotin-conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation, a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 8 hours with TNF alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 ug/ml) in tissue cu
|IHC-Fr||Use at an assay dependent dilution.|
|Flow Cyt||Use at an assay dependent dilution.|
ab7219 has not yet been referenced specifically in any publications.
Publishing research using ab7219 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"