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Product Name
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VCAM1 antibody [1G11B1]
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See all VCAM1 antibodies (20)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [1G11B1] to VCAM1
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Immunogen
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Full length protein (Human).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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Flow Cyt, ICC, IHC-Fr, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
The antibody is extremely useful for staining of VCAM-1 expressing endothelial cells.
It permits staining of in vitro cultured cells and frozen tissue sections.
The antibody can be used for flow cytometry and immunoprecipitation.
In vitro cultured cells can be fixed with 1% paraformaldehyde. Tissue sections should be fixed for 10 min in pure acetone followed by incubation for 10 min in chloroform.
Incubation with a pretested dilution of the antibody is advised followed by a biotin-conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin.
For selection of the most useful dilution in a given situation, a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 8 hours with TNF alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 ug/ml) in tissue culture medium at 37°C.
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Positive control
(see definition)
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Lymph nodes can be used as positive control.
As negative control it is advised to use as first antibody a control murine IgG1 antibody.
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Cellular localization
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Membrane; Single pass type I membrane protein.
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Research areas
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Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Neural Signal Transduction
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Vascular
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Relevance
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VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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1G11B1
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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PBS with 0.2% BSA, 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VCAM1 antibody [1G11B1] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to VCAM1
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VCAM1 antibody [1G11B1] - more information
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