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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Vascular
Overview
Product name
Anti-VCAM1 antibody [1G11B1]
See all VCAM1 products (29) ...
Description
Mouse monoclonal [1G11B1] to VCAM1
Specificity
The antibody is extremely useful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. The antibody can be used for flow cytometry and immunoprecipitation.
Tested applications
ICC, IP, IHC-Fr, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length protein (Human).
Positive control
Lymph nodes can be used as positive control. As negative control it is advised to use as first antibody a control murine IgG1 antibody.
General notes
In vitro cultured cells can be fixed with 1% paraformaldehyde. Tissue sections should be fixed for 10 min in pure acetone followed by incubation for 10 min in chloroform.
Incubation with a pretested dilution of the antibody is advised followed by a biotin-conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin.
For selection of the most useful dilution in a given situation, a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 8 hours with TNF alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 ug/ml) in tissue culture medium at 37°C.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS with 0.2% BSA, 0.1% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
1G11B1
Isotype
IgG1
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Neural Signal Transduction
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Vascular
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution. (The antibody is extremely useful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. The antibody can be used for flow cytometry and immunoprecipitation.In vitro cultured cells can be fixed with 1% paraformaldehyde. Tissue sections should be fixed for 10 min in pure acetone followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised followed by a biotin-conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation, a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 8 hours with TNF alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 ug/ml) in tissu
IHC-Fr: Use at an assay dependent dilution.
-
Flow Cyt: Use at an assay dependent dilution.
Target
Function
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Tissue specificity
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
Sequence similarities
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Domain
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
Post-translational
modifications
Sialoglycoprotein.
Cellular localization
Membrane.
Target information above from: UniProt accessionP19320
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 106 antibody
- CD106 antibody
- CD106 Antigen antibody
see all
Database links
- Entrez Gene: 7412 Human
- Omim: 192225 Human
- SwissProt: P19320 Human
- Unigene: 109225 Human
References for Anti-VCAM1 antibody [1G11B1] (ab7219)
ab7219 has not yet been referenced specifically in any publications.
Publishing research using ab7219? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"