Overview

  • Product nameAnti-VCAM1 antibody [324]
    See all VCAM1 primary antibodies
  • Description
    Rat monoclonal [324] to VCAM1
  • Tested applicationsWB, ICC, IHC (Methanol fixed), IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice.

  • Positive control
    • Brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab78712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 20 µg/ml. Predicted molecular weight: 81 kDa.
ICC Use a concentration of 5 - 10 µg/ml.
IHC (Methanol fixed) Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.

Target

  • FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications
    Sialoglycoprotein.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 106 antibody
    • CD106 antibody
    • CD106 Antigen antibody
    • DKFZp779G2333 antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

References for Anti-VCAM1 antibody [324] (ab78712)

This product has been referenced in:
  • Dai C  et al. Apolipoprotein A-I attenuates ovalbumin-induced neutrophilic airway inflammation via a granulocyte colony-stimulating factor-dependent mechanism. Am J Respir Cell Mol Biol 47:186-95 (2012). WB ; Mouse . Read more (PubMed: 22427535) »

See 1 Publication for this product

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Thank you very much for your interest in ab our VCAM1 antibodies.


I have checked our IHC-P-guaranteed VCAM1 antibodies for their potential cross-reactivity with rabbit tissue, and based on the immunogen sequence similarity of 86% I may...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"