Overview

  • Product nameAnti-VCAM1 antibody [6G9]See all VCAM1 primary antibodies ...
  • Description
    Mouse monoclonal [6G9] to VCAM1
  • Tested applicationsWB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Rabbit, Human
  • Immunogen

    Purified recombinant fragment of Human VCAM1 expressed in E. Coli.

  • Positive control
    • HUVEC and EC cell lysate. Human lung cancer and colon cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab98954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 81 kDa.
IHC-P 1/200 - 1/1000.
ELISA 1/10000.

Target

  • FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications
    Sialoglycoprotein.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

Anti-VCAM1 antibody [6G9] images

  • All lanes : Anti-VCAM1 antibody [6G9] (ab98954) at 1/500 dilution

    Lane 1 : HUVEC cell lysate
    Lane 2 : EC cell lysate


    Predicted band size : 81 kDa
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue (1) and colon cancer tissue (2) using ab98954 at 1/200 dilution with DAB staining.

References for Anti-VCAM1 antibody [6G9] (ab98954)

This product has been referenced in:
  • Valero-Muñoz M  et al. Relevance of vascular peroxisome proliferator-activated receptor ? coactivator-1a to molecular alterations in atherosclerosis. Exp Physiol 98:999-1008 (2013). Read more (PubMed: 23335007) »
  • Delfortrie S  et al. Egfl7 promotes tumor escape from immunity by repressing endothelial cell activation. Cancer Res 71:7176-86 (2011). IHC-P ; Human . Read more (PubMed: 22037871) »

See all 2 Publications for this product

Product Wall


I have contacted the lab and will let you know what explanation they have for the 55 kDa band.

As for the reference I found in our internal notes, it says: Researchers have shown detecting a band at 43kda which is a GPI-VCAM1. http://www....

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Please check the online datasheets of all antibodies. You will find the required information.

Thanks!

Thank you very much for contacting us.


The possible rabbit specific antibodies are available as;


ICAM1: the rabbit protein sequence (http://www.uniprot.org/uniprot/Q75T02) does not show any homology with human protein (http:...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (vascular tissue)
Loading amount 40 µg
Specification vascular tissue
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 10 2012

¡Muchas gracias por la información !

Esperamos entonces tus resultados para poder completar la información de estos anticuerpos.

¡Me alegra saber que están saliendo buenos resultados!

Si ti...

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Thank you very much for your interest in ab98954.

To our knowledge,ab98954 has not been experimentallytested yetin rabbitbut the immunogen shows a very high homology to rabbit.

Therefore, I can offer a discount off a future purchas...

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Gracias por u respuesta.

Me alegro que hayáis conseguido optimizar los resultados de ab25072. Para mandarnos los resultados en conejo de éste y otros anticuerpos, seguid las instrucciones indicadas en el siguiente link:
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Thank you very much for your interest in ab our VCAM1 antibodies.


I have checked our IHC-P-guaranteed VCAM1 antibodies for their potential cross-reactivity with rabbit tissue, and based on the immunogen sequence similarity of 86% I may...

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This product is indeed out of Abpromise guarantee period and I would really encourage you to let your customer know about the guarantee period in your region. As we are retesting this product in order to determine the reason of 50 kDa band so w...

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Thanks you for your email. I understand customer's concerns. The predicted band size of VCAM1 is 81kDa however due to protein glycosylation the observed band size should be near 100 kDa. This is what we are observing with different antibodies an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"