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Immunohistochemistry (Frozen sections) - VCAM1 antibody [M/K-2] (ab19569)
![Immunohistochemistry (Frozen sections) - VCAM1 antibody [M/K-2] (ab19569)](/ps/datasheet/images/19/ab19569/VCAM1-Primary-antibodies-ab19569-2.jpg)
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Product Name
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VCAM1 antibody [M/K-2]
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See all VCAM1 antibodies (20)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [M/K-2] to VCAM1
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Immunogen
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BALB/3T3 and +/+ 2.4 cells.
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Reacts with
(species key)
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Ms
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Specificity
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Present on the +/+ 2.4 stromal cell clone, large cells in bone marrow, and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. Reacts with all known isoforms of murine VCAM-1. Murine vascular adhesion molecule (VCAM1), single chain glycoprotein of Mr100-kDa (under reducing conditions).
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Tested applications
(see key)
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FuncS, ICC/IF, IHC-Fr
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Abreviews
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Application notes
(see key)
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Recommended dilutions
FuncS: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
Studies of cell-cell interaction.
Cell adhesion assays.
Inhibition of lymphopoiesis in Whitlock-Witte long term marrow cultures.
Immunoprecipitation of the VCAM1 integrin complex.
Is unsuitable for IHC-P.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Membrane; Single pass type I membrane protein.
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Research areas
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Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Neural Signal Transduction
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Vascular
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Relevance
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VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 22329 (Mouse)
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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M/K-2
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: 10mM Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VCAM1 antibody [M/K-2] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VCAM1 antibody [M/K-2] images, references, products related to ab19569 and other tools.
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VCAM1 antibody [M/K-2] images:
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 Immunohistochemistry (Frozen sections) - VCAM1 antibody [M/K-2] (ab19569)
ab19569 at a 1/1000 diution staining VCAM1 in mouse brain tissue by Immunohistochemistry (frozen sections), incubated for 2 hours at 20°C. PFA fixed. Blocked with 10% serum for 1 hour at 20°C. Secondary used at 1/100 ab6733 Rabbit polyclonal to Rat IgG - H&L (Biotin).DAB staining (brown colour). Counterstain is Chresyl violet. This image was kindly supplied by Dr Thomas Weissensteiner by Abreview
See Abreview
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Search PubMed (MEDLINE) for references to VCAM1
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VCAM1 antibody [M/K-2] - more information
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Customer reviews (feedback) regarding VCAM1 antibody [M/K-2] |
Customer FAQs regarding VCAM1 antibody [M/K-2] |
Protocols for VCAM1 antibody [M/K-2] |
Price and availability of products related to VCAM1 antibody [M/K-2] |
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VCAM1 antibody [M/K-2] - related products:
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VCAM1 antibody [M/K-2] - other tools:
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Contact Abcam with a Technical Enquiry about VCAM1 antibody [M/K-2] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (Frozen sections) - VCAM1 antibody [M/K-2] (ab19569)](/index.html?datasheet=19569#VCAM1-Primary-antibodies-ab19569-2.jpg)
