The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µg for 105-8 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
Staining of rat bone marrow cells with 0.25 µg of Mouse IgG1 (open histogram) or 0.25 µg of ab95541 (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE . Cells in the large scatter population were used for analysis.