The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µg for 105-8 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
Flow Cytometry - VCAM1 antibody [MR106 ] (ab95541)Staining of rat bone marrow cells with 0.25 µg of Mouse IgG1 (open histogram) or 0.25 µg of ab95541 (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE . Cells in the large scatter population were used for analysis.
References for Anti-VCAM1 antibody [MR106] (ab95541)
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