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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Vascular
Overview
Product name
Anti-VCAM1 antibody [MVCAM.A(429)]
See all VCAM1 products (29) ...
Description
Rat monoclonal [MVCAM.A(429)] to VCAM1
Tested applications
Flow Cyt, IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Tissue / cell preparation (Mouse) - Stromal cell line PA6.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide.
Constituents: PBS, pH 7.4.
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody was purified from tissue culture supernatant by affinity chromatography.
Primary antibody notes
This antibody is reported to partially block VCAM1 mediated functions.
Clonality
Monoclonal
Clone number
MVCAM.A(429)
Myeloma
P3x63-Ag8.653
Isotype
IgG2a
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Neural Signal Transduction
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Vascular
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/100 - 1/200.(Use 10µl of the suggested working dilution to label 106 cells in 100µl.)
IHC-Fr: Use at an assay dependent dilution.
Target
Function
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Tissue specificity
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
Sequence similarities
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Domain
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
Post-translational
modifications
Sialoglycoprotein.
Cellular localization
Membrane.
Target information above from: UniProt accessionP19320
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 106 antibody
- CD106 antibody
- CD106 Antigen antibody
see all
Database links
- Entrez Gene: 22329 Mouse
- SwissProt: P29533 Mouse
- Unigene: 440909 Mouse
- Unigene: 76649 Mouse
References for Anti-VCAM1 antibody [MVCAM.A(429)] (ab27560)
This product has been referenced in:
- Mikkelsen HBet al. The macrophage system in the intestinal muscularis externa during inflammation: an immunohistochemical and quantitative study of osteopetrotic mice. Histochem Cell Biol 130:363-73 (2008). IHC-Fr; Mouse.Read more (PubMed: 18392842) »
See 1 publication for this product
Publishing research using ab27560? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"