Overview

  • Product nameAnti-VCAM1 antibody [STA]See all VCAM1 primary antibodies ...
  • Description
    Mouse monoclonal [STA] to VCAM1
  • Tested applicationsIP, ELISA, IHC-Fr, Flow Cyt more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human DS6 T cell line

  • Positive control
    • Flow Cyt: TNF-alpha activated HUVEC cells

Properties

Applications

Our Abpromise guarantee covers the use of ab95139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution. Acetone fixation
Flow Cyt Use a concentration of 6 µg/ml.

Target

  • FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications
    Sialoglycoprotein.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P19320 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

References for Anti-VCAM1 antibody [STA] (ab95139)

This product has been referenced in:
  • Yen YT  et al. Modeling the early events of severe acute respiratory syndrome coronavirus infection in vitro. J Virol 80:2684-93 (2006). Read more (PubMed: 16501078) »

See 1 Publication for this product

Product Wall

Abreviews
Application ELISA
Sample Human Serum (cardiovascular biomarker)
Specification cardiovascular biomarker
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Type Sandwich (Detection)
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Verified customer

Submitted Mar 13 2013

Thank you for contacting us.

Effectively all of the products mentioned are suitable, and therefore are guaranteed for IHC in frozen sections in the species stated on their datasheets.

However, instead of using PFA as a fixative, I w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"