Anti-VCAM1 antibody (ab106777)

Overview

  • Product nameAnti-VCAM1 antibodySee all VCAM1 primary antibodies ...
  • Description
    Rabbit polyclonal to VCAM1
  • Tested applicationsWB, ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit
  • Immunogen

    Recombinant fragment, corresponding to amino acids 195-474 of Human VCAM1 (BC068490).

  • Positive control
    • HeLa cell lysate, Human fetal spleen, kidney and heart lysates; Human fetal skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab106777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Predicted molecular weight: 81 kDa.
ELISA 1/20000 - 1/80000.
IHC-P 1/100 - 1/500.

Target

  • FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications
    Sialoglycoprotein.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P19320 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

Anti-VCAM1 antibody images

  • All lanes : Anti-VCAM1 antibody (ab106777) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal spleen lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal heart lysate


    Predicted band size : 81 kDa
  • ab106777 at 1/100 dilution, staining VCAM1 in Human fetal skin by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

References for Anti-VCAM1 antibody (ab106777)

ab106777 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application ELISA
Sample Human Serum (cardiovascular biomarker)
Specification cardiovascular biomarker
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Type Sandwich (Capture)
Username

Abcam user community

Verified customer

Submitted Mar 13 2013

Reply to CCE ******* and CCE ******** as I assume both complaints are from the same customer.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear both antibodies ab41532 and ab106777 are not provi...

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Thank you for your reply regarding ab106777.


I am happy to hear that this antibody worked successfully for you in rat. In order to validate the discount code, please submit an Abreview through our website:


http://www.abcam.c...

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DISCOUNT CODE: ***********
Expiration date: *************

I am very pleased to hear you would like to accept our offer and test ab106777 in rat tissue. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To rede...

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Thank you very much for your interest in ab106777.

To our knowledge, ab106777 has not been tested in rat. Therefore, I can offer a discount off a future purchase if you buy ab106777 now, test it in rat and submit feedback to us in the form o...

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I can confirm that we didn't test the antibody (ab106777: Anti-VCAM1) in Rabbit tissue, as soon as we have further information we will update our on-line datasheet accordingly.

Thank you for your interest.



The reactivity of this antibody (ab2213) with rabbit samples on Western blot application is probable but it has not been tested yet.



Hope this will be useful for you!

Abreviews
Application Western blot
Sample Cow Cell lysate - whole cell (Primary Endothelial cells)
Loading amount 5 µg
Specification Primary Endothelial cells
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. S Maalouf

Verified customer

Submitted Dec 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"