![Immunocytochemistry/ Immunofluorescence - VCP antibody [3E8DC11 ] (ab110308) image](#VCP-Primary-antibodies-ab110308-1.jpg)
![Immunoprecipitation - VCP antibody [3E8DC11 ] (ab110308) image](#VCP-Primary-antibodies-ab110308-2.jpg)
![Flow Cytometry - VCP antibody [3E8DC11 ] (ab110308) image](#VCP-Primary-antibodies-ab110308-3.jpg)
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Product name
Anti-VCP antibody [3E8DC11 ]
See all VCP products (12) ...
Description
Mouse monoclonal [3E8DC11 ] to VCP
Tested applications
In-Cell ELISA, ICC/IF, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Sucrose gradient fraction 4 from Human liver mitochondria.
Positive control
ICC/IF: Human HDFn cells IP: Human, Rat, and Mouse liver samples; HepG2 cultured cell lysate Flow Cyt: HL-60 cells
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
The purity of ab110308 is near homogeneity, as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
3E8DC11
Isotype
IgG1
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
In-Cell ELISA: Use a concentration of 2 µg/ml. (0.2 µg/well)
ICC/IF: Use a concentration of 2 µg/ml.
Flow Cyt: Use a concentration of 1 µg/ml.
IP: Use at an assay dependent dilution.
Target
Function
Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A.
Involvement in disease
Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) [MIM:167320]; also known as muscular dystrophy, limb-girdle, with Paget disease of bone or pagetoid amyotrophic lateral sclerosis or pagetoid neuroskeletal syndrome or lower motor neuron degeneration with Paget-like bone disease. IBMPFD features adult-onset proximal and distal muscle weakness (clinically resembling limb girdle muscular dystrophy), early-onset Paget disease of bone in most cases and premature frontotemporal dementia.
Sequence similarities
Belongs to the AAA ATPase family.
Post-translational
modifications
Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated.
Cellular localization
Cytoplasm > cytosol. Nucleus. Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
Target information above from: UniProt accessionP55072
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 15S Mg(2+) ATPase p97 subunit antibody
- 15S Mg(2+)-ATPase p97 subunit antibody
- ATPase p97 antibody
see all
Database links
- Entrez Gene: 7415 Human
- Entrez Gene: 269523 Mouse
- Entrez Gene: 269523 Mouse
- Entrez Gene: 116643 Rat
- Entrez Gene: 116643 Rat
- Omim: 601023 Human
- SwissProt: P55072 Human
- SwissProt: Q01853 Mouse
see all
Anti-VCP antibody [3E8DC11 ] images:
Immunocytochemistry/ Immunofluorescence - VCP antibody [3E8DC11 ] (ab110308)
![Immunocytochemistry/ Immunofluorescence - VCP antibody [3E8DC11 ] (ab110308)](/ps/datasheet/images/110/ab110308/VCP-Primary-antibodies-ab110308-1.jpg)
Immunocytochemistry image of ab110308-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab110308 at 2 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates mainly in nucleus and cytoplasm.
Immunoprecipitation - VCP antibody [3E8DC11 ] (ab110308)
![Immunoprecipitation - VCP antibody [3E8DC11 ] (ab110308)](/ps/datasheet/images/110/ab110308/VCP-Primary-antibodies-ab110308-2.jpg)
ab110308 pulls down the 89 kDa VCP protein in Human (lane 1), Rat (lane 2), and Mouse (lane 3) liver samples and Human HepG2 cultured cell lysate (lane 4). The identity of this protein was confirmed by mass spectrometry. This gel was stained with sypro ruby gel stain.
Flow Cytometry - VCP antibody [3E8DC11 ] (ab110308)
![Flow Cytometry - VCP antibody [3E8DC11 ] (ab110308)](/ps/datasheet/images/110/ab110308/VCP-Primary-antibodies-ab110308-3.jpg)
HL-60 cells were stained with 1 µg/mL ab110308 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
References for Anti-VCP antibody [3E8DC11 ] (ab110308)
ab110308 has not yet been referenced specifically in any publications.
Publishing research using ab110308? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"