Anti-VCP antibody (ab111740)
Key features and details
- Rabbit polyclonal to VCP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-VCP antibody
See all VCP primary antibodies -
Description
Rabbit polyclonal to VCP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish -
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 290 and 507 of human VCP (P55072)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/20000. Predicted molecular weight: 89 kDa.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/20000. Predicted molecular weight: 89 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A. -
Involvement in disease
Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) [MIM:167320]; also known as muscular dystrophy, limb-girdle, with Paget disease of bone or pagetoid amyotrophic lateral sclerosis or pagetoid neuroskeletal syndrome or lower motor neuron degeneration with Paget-like bone disease. IBMPFD features adult-onset proximal and distal muscle weakness (clinically resembling limb girdle muscular dystrophy), early-onset Paget disease of bone in most cases and premature frontotemporal dementia. -
Sequence similarities
Belongs to the AAA ATPase family. -
Post-translational
modificationsPhosphorylated by tyrosine kinases in response to T-cell antigen receptor activation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients. - Information by UniProt
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Database links
- Entrez Gene: 507345 Cow
- Entrez Gene: 7415 Human
- Entrez Gene: 269523 Mouse
- Entrez Gene: 397524 Pig
- Entrez Gene: 116643 Rat
- Entrez Gene: 380491 Xenopus laevis
- Entrez Gene: 327197 Zebrafish
- Omim: 601023 Human
see all -
Alternative names
- 15S Mg(2+) ATPase p97 subunit antibody
- 15S Mg(2+)-ATPase p97 subunit antibody
- ALS14 antibody
see all
Images
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All lanes : Anti-VCP antibody (ab111740) at 1/1000 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : U87-MG whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 89 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of VCP in paraformaldehyde fixed MCF7, using ab111740 at a 1/500 dilution. Lower image shows cells costained with Hoechst 33342.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab111740 has been referenced in 1 publication.
- Jongkamonwiwat N et al. Noise Exposures Causing Hearing Loss Generate Proteotoxic Stress and Activate the Proteostasis Network. Cell Rep 33:108431 (2020). PubMed: 33238128