Anti-VDAC/Porin antibody - Mitochondrial Marker (ab61273)

Overview

• Product nameAnti-VDAC/Porin antibody - Mitochondrial MarkerSee all VDAC/Porin primary antibodies ...
• Description
  Mouse monoclonal to VDAC/Porin - Mitochondrial Marker
• Tested applicationsWB, Flow Cyt, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Cynomolgus Monkey, Rhesus monkey, Opossum
• Immunogen

  Recombinant fragment with tag: MAVPPTYADL GKSARDVFTK GYGFGLIKLD LKTKSENGLE FTSSGSANTE TTKVTGSLET KYRWTEYGLT FTEKWNTDNT LGTEITVEDQ LARGLKLTFD , corresponding to amino acids 1-100 of Human VDAC1 / Porin

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• IsotypeIgG2a
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Types of disease
  • Cancer

  • Cancer
  • Cancer Metabolism
  • Cellular metabolic process

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Signal Transduction
  • Metabolism
  • Plasma Membrane
  • Channels

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

Applications

Target

• FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
• Tissue specificityHeart, liver and skeletal muscle.
• Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
• DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
• Cellular localizationMitochondrion outer membrane. Cell membrane.

Target information above from: UniProt accession P21796 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 416320 Chicken
  • Entrez Gene: 282119 Cow
  • Entrez Gene: 7416 Human
  • Entrez Gene: 7416 Human
  • Entrez Gene: 7416 Human
  • Entrez Gene: 7416 Human
  • Entrez Gene: 22333 Mouse
  • Entrez Gene: 397010 Pig

  • Entrez Gene: 100008751 Rabbit
  • Entrez Gene: 83529 Rat
  • Entrez Gene: 380376 Xenopus laevis
  • Entrez Gene: 334582 Zebrafish
  • Omim: 604492 Human
  • SwissProt: P45879 Cow
  • SwissProt: P21796 Human
  • SwissProt: P21796 Human
  • SwissProt: P21796 Human
  • SwissProt: P21796 Human
  • SwissProt: Q60932 Mouse
  • SwissProt: Q9MZ16 Pig
  • SwissProt: Q9TT15 Rabbit
  • SwissProt: Q9Z2L0 Rat
  • Unigene: 519320 Human
  • Unigene: 709506 Human
  • Unigene: 3555 Mouse
  • Unigene: 54594 Rat

  see all

• Alternative names
  N2441 antibody OMP2 antibody POR1 antibody

  hVDAC1 antibodyMGC111064 antibodyOuter mitochondrial membrane protein porin 1 antibodyPlasmalemmal porin antibodyPorin 31 HL antibodyPorin 31HL antibodyPorin 31HM antibodyVDAC 1 antibodyVDAC antibodyVDAC-1 antibodyVDAC1 antibodyVDAC1_HUMAN antibodyVoltage dependent anion channel 1 antibodyVoltage dependent anion selective channel protein 1 antibodyVoltage-dependent anion-selective channel protein 1 antibodyYNL055C antibodyYNL2441C antibody

  see all

Anti-VDAC/Porin antibody - Mitochondrial Marker images

• 

  Western blot - VDAC1 / Porin antibody (ab61273)
  
  
  Predicted band size : 31 kDa
  
• 

  Flow Cytometry - Anti-VDAC/Porin antibody - Mitochondrial Marker (ab61273)
  Overlay histogram showing HepG2 cells stained with ab61273 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab61273, 0.5µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.