Anti-VDAC/Porin antibody - Mitochondrial Marker (ab76441)

Overview

• Product nameAnti-VDAC/Porin antibody - Mitochondrial MarkerSee all VDAC/Porin primary antibodies ...
• Description
  Mouse monoclonal to VDAC/Porin - Mitochondrial Marker
• Tested applicationsELISA, WB more details
• Species reactivity
  Reacts with: Recombinant Fragment
  Predicted to work with: Human
• Immunogen

  Recombinant fragment (Human) VDAC/Porin (VDAC1) protein 156 a.a. ~ 276 a.a) with a tag. Total MW is 39kDa, MW of tag alone is 26 kDa.

• Positive controlimmunogen

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Types of disease
  • Cancer

  • Cancer
  • Cancer Metabolism
  • Cellular metabolic process

  • Signal Transduction
  • Metabolism
  • Plasma Membrane
  • Channels

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

  • Neuroscience
  • Neurotransmission
  • Receptors / Channels
  • More Ion Channels

  • Cell Biology
  • Apoptosis
  • Mitochondrial

Applications

Target

• FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
• Tissue specificityHeart, liver and skeletal muscle.
• Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
• DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
• Cellular localizationMitochondrion outer membrane. Cell membrane.

Target information above from: UniProt accession P21796 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7416 Human
  • Entrez Gene: 7416 Human
  • Entrez Gene: 7416 Human
  • Entrez Gene: 7416 Human
  • Omim: 604492 Human
  • SwissProt: P21796 Human
  • SwissProt: P21796 Human
  • SwissProt: P21796 Human

  • SwissProt: P21796 Human
  • Unigene: 519320 Human
  • Unigene: 709506 Human

  see all

• Alternative names
  N2441 antibody OMP2 antibody POR1 antibody

  hVDAC1 antibodyOuter mitochondrial membrane protein porin 1 antibodyPlasmalemmal porin antibodyPorin 31HL antibodyPorin 31HM antibodyVDAC antibodyVDAC-1 antibodyVdac1 antibodyVDAC1_HUMAN antibodyVoltage dependent anion channel 1 antibodyVoltage dependent anion selective channel protein 1 antibodyVoltage-dependent anion-selective channel protein 1 antibodyYNL055C antibodyYNL2441C antibody

  see all

Anti-VDAC/Porin antibody - Mitochondrial Marker images

• 

  Western blot - VDAC/Porin antibody (ab76441)
  Anti-VDAC/Porin antibody - Mitochondrial Marker (ab76441) at 1/500 dilution + immunogen at 0.1 µg
  
  Secondary
  Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
  
  Predicted band size : 31 kDa
  Observed band size : 39 kDa (why is the actual band size different from the predicted?)